Acquity uplc protein beh c4 reverse phase column

Manufactured by Waters Corporation

The ACQUITY UPLC Protein BEH C4 reverse-phase column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of proteins. It utilizes the BEH (Ethylene Bridged Hybrid) particle technology to provide efficient and high-resolution protein separations.

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G2 s qtof, by Waters Corporation (2 mentions)

2 protocols using acquity uplc protein beh c4 reverse phase column

Protein Characterization by UPLC-MS

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Mass spectra of intact proteins were obtained using a Xevo G2-S QTOF on a Waters ACQUITY UPLC Protein BEH C4 reverse-phase column (300 A, 1.7 μm, 2.1 mm × 50 mm). An acetonitrile gradient from 5 %-95 % was used with 0.1 % formic acid, over a run time of 5 min and a constant flow rate of 0.5 mL/min at 40 °C. The spectra were deconvoluted using MaxEnt1.

Ghelichkhani F., Gonzalez F.A., Kapitonova M.A, & Rozovsky S. (2023). Selenoprotein S Interacts with the Replication and Transcription Complex of SARS-CoV-2 by Binding nsp7. Journal of Molecular Biology, 435(8), 168008.

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Mass Spectrometry of Intact Proteins

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Mass spectra of intact proteins were obtained using a Xevo G2-S QTOF on a Waters ACQUITY UPLC Protein BEH C4 reverse-phase column (300 Å, 1.7 μm, 2.1 mm x 150 mm). An acetonitrile gradient from 5%−95% was used with 0.1% formic acid, over a run time of 5 min and constant flow rate of 0.5 mL/min at room temperature. Spectra were acquired from m/z 350 to 2000, at a rate of 1 sec/scan. Each analysis was 25 min under constant flow rate of 0.2 mL/min at room temperature. Data were acquired from m/z 350 to 2500, at a rate of 1 sec/scan. The spectra were deconvoluted using maximum entropy in MassLynx. All data are reported within 30 ppm.

Liu J., Ekanayake O., Santoleri D., Walker K, & Rozovsky S. (2019). Efficient generation of hydrazides in proteins by RadA split intein. Chembiochem : a European journal of chemical biology, 21(3), 346-352.

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