AMPKα (2532), phosphor-AMPKα (T172) (2531), ACC (3662) and phosphor-ACC (S79) (11818) antibodies were purchased from Cell Signaling Technology. UCP1 (ab23841) antibody was purchased from Abcam. β-actin (A5316) antibody was purchased from Sigma-Aldrich. Mouse IgG (NA931V) and rabbit IgG (NA934V) HRP-linked whole antibodies were purchased from GE Healthcare. To generate an anti-HDAC11 antibody, a peptide corresponding to amino acids 179–193 of HDAC11 (DLDAHQGNGHERDFM) was injected subcutaneously into New Zealand white rabbits (Alpha Diagnostic, Inc.). The resulting antibody was immune-affinity purified on a peptide column with Affi-gel agarose (Bio-Rad). Specificity of this anti-HDAC11 antibody was confirmed using brain tissue extracts from HDAC11 KO and WT mice. Oil red O (O0625) and oleic acid (O3008) were purchased from Sigma-Aldrich. AdipoRon (15941) was purchased from Cayman Chemical, and Insulin-Transferrin-Selenium (41400–045) was from Thermo Fisher Scientific.
Affi gel agarose
Manufactured by Bio-Rad
Affi-gel agarose is a laboratory product designed for biological and biochemical applications. It is an agarose-based material used for a variety of separation and purification techniques. The core function of Affi-gel agarose is to provide a matrix for the immobilization of proteins, enzymes, and other biomolecules, enabling their isolation and purification from complex mixtures.
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Lab products found in correlation
2 protocols using affi gel agarose
1
Antibodies and Reagents for AMPK Signaling
2
HDAC and PU.1 Expression Plasmids
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Flag-tagged HDAC expression plasmids were previously described (Radhakrishnan et al, 2015 (link)). Similarly, PU.1 expression plasmids and PU.1 deletion constructs were previously described (Zhong et al, 2005 (link)). HDAC1 antibody was described (Tsai et al, 2000 (link)). Flag (clone M2) and HA (SAB1305536) antibodies were purchased from Sigma-Aldrich. IgG control (sc-2027) antibody was purchased from Santa Cruz Biotechnology. MBP (SMI 99) antibody was purchased from BioLegend. Anti-Iba1 (ab5076) was purchased from Abcam. To generate an anti-HDAC11 antibody, a peptide corresponding to amino acids 179–193 of HDAC11 (DLDAHQGNGHERDFM) coupled to keyhole limpet hemocyanin was injected subcutaneously into New Zealand white rabbits. The resulting antibody was immunoaffinity purified on a peptide column with Affi-gel agarose (Bio-Rad). Specificity of this anti-HDAC11 antibody was confirmed using brain tissue extracts from HDAC11 KO and WT mice.
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