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Antibody Sources for Protein Analysis

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The antibodies used for this study were purchased from the following commercial sources: anti-LRPPRC (Western blot; sc-166178, Santa Cruz, Texas, USA); anti-LRPPRC (co-immunoprecipitation [co-IP] and IHC; 21175-1-AP, Proteintech, Chicago, USA); anti-SLIRP (Western blot, co-IP and IHC; 26006-1-AP, Proteintech, Chicago, USA); anti-Flag (Western blot and co-IP; 8146, CST, Boston, USA); anti-FOXM1 (Western blot and IHC; 13147-AP, Proteintech, Chicago, USA); anti-PRDX3 (Western blot and IHC; 10664-AP, Proteintech, Chicago, USA); anti-MnSOD (Western blot; S5069, Sigma Aldrich, USA); anti-Catalase (Western blot; 21260-1-AP, Proteintech, Chicago, USA); anti-Cleaved CASP9; anti-Cleaved CASP3; anti-Cleaved PARP (Western blot; 9929, CST, Boston, USA); anti-CASP8 (Western blot; 4790, CST, Boston, USA); anti-BCL2 (Western blot; 4223, CST, Boston, USA); anti-BAX (Western blot; 5023, CST, Boston, USA); and anti-α-Tubulin (Western blot; 66031-1-Ig, Proteintech, Chicago, USA).
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2

Immunoblotting Analysis of EMT-related Proteins

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Whole cell lysates were prepared in RIPA buffer containing protease inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA, USA).19 (link) Immunoblotting was performed with anti-MUC1-C (#MA5-11202, 1:100; Thermo Fisher Scientific), anti-ZEB1 (#3396, 1:1000 dilution; Cell Signaling Technology (CST), Danvers, MA, USA), anti-TWIST1 (ab50887, 1:1000 dilution; Abcam, Waltham, MA, USA), anti-BMI1 (#6964, 1:1000 dilution; CST), anti-MYC (#ab32072, 1:1000 dilution; Abcam), anti-SOX2 (#3579, 1:1000 dilution; CST), anti-GLUT1 (#ab115730, 1:100000 dilution; Abcam), anti-HK2 (#2867, 1:1000 dilution; CST), anti-SDHD (#NBP2-83505, 1:1000, NOVUS Biologicals, Centennial, CO, USA), anti-cytochrome c (#10993, 1:4000 dilution; Proteintech; Rosemont, IL, USA), anti-ND1 (#19703, 1:1000 dilution; Proteintech), anti-COX2 (#55070, 1:5000 dilution; Proteintech), anti-TFAM (#8076, 1:1000 dilution; CST), anti-TFB2M (#24411, 1:500 dilution; Proteintech), anti-mTERF3 (#ab230232, 1:1000 dilution; Abcam), anti-SOD2 (#13141, 1:1000 dilution; CST), anti-PRDX3 (#10664, 1:2000 dilution; Proteintech), and anti-β-actin (A5441; 1:50000 dilution; Sigma, St. Louis, MO, USA).
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