Broccoli seeds (BHL106) were obtained from the Vegetable Research Center of the Beijing Academy of Agricultural and Forestry Sciences. Methanol, formic acid, ammonium acetate, acetonitrile, Sinigrin (SIN), Gluconapin (NAP), Progoitrin (PRO), Glucotropaeolin (TRO), Glucoerucin (ERU), Gluconasturtiin (NAS), Glucoraphenin (RAE), Glucobrassicin (GBC), Glucoalyssin (ALY), 4-MethoxyGlucobrassicin (4ME), NeoGlucobrassicin (NEO), and glucoraphanin were purchased from PhytoLab GmbH & Co. KG (Vestenbergsgreuth, Middle Franconia, Germany). The purity of all chemical substances was >99%. Individual stock solutions of the 13 GSLs were prepared in 70% methanol. Ultrapure deionized water (18.2 MΩ) was prepared using Milli-Q purification systems (Millipore, MA, USA).
Sinigrin
Manufactured by Phytolab
Sourced in Germany
Sinigrin is a glucosinolate compound found in various cruciferous vegetables. It is a naturally occurring compound that can be extracted and used as a laboratory reagent. Sinigrin serves as a precursor for the production of other chemical compounds and is used in various research and analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
Milli q purification system, by Merck Group (2 mentions)
Gluconapin, by Phytolab (2 mentions)
Glucoerucin, by Phytolab (2 mentions)
Progoitrin, by Phytolab (2 mentions)
Gluconasturtiin, by Phytolab (2 mentions)
Glucoraphenin, by Phytolab (2 mentions)
Glucobrassicin, by Phytolab (2 mentions)
Glucoraphanin, by Phytolab (2 mentions)
Glucotropaeolin, by Phytolab (2 mentions)
Hplc grade methanol, by Thermo Fisher Scientific (1 mentions)
Lc ms grade formic acid, by Thermo Fisher Scientific (1 mentions)
Lc ms grade methanol, by Thermo Fisher Scientific (1 mentions)
Glucobrassicanapin, by Phytolab (1 mentions)
3 protocols using sinigrin
1
Quantification of Glucosinolates in Broccoli
2
Quantitative Analysis of Glucosinolates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
High-purity (>98%) GSL standards were purchased from PhytoLab GmbH & Co. KG (Vestenbergsgreuth, Middle Franconia, Germany): sinigrin (SIN), gluconapin (NAP), glucobrassicanapin (GBN), progoitrin (PRO), glucoerucin (ERU), glucoraphenin (RAE), glucoraphanin (RAA), glucobrassicin (GBC), glucotropaeolin (TRO) and gluconasturtiin (NAS). 4-Methoxyglucobrassicin (4ME) and neoglucobrassicin (NEO) were purchased from Extrasynthese (Z.I Lyon Nord, Genay Cedex France) and glucoalyssin (ALY) was purchased from Shanghai ZZBIO Co., Ltd. (Shanghai, China). HPLC-grade methanol, LC/MS-grade methanol and LC/MS-grade formic acid were supplied by Fisher Scientific (Pittsburgh, PA, USA). Ultrapure water was obtained using Milli-Q purification systems (Millipore, MA, USA). Individual stock solutions of the 13 GSLs were prepared in 70% methanol.
3
Metabolite Extraction and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
5-days after infiltration, four leaf disks of 1 cm diameter were harvested from each infiltrated leaf and weighed. The total metabolites were extracted using 85% methanol and the extract was diluted 10 times (1 vol. extract :9 vol. stock solution) with a stock solution containing 10 μg/ml 13 C-, 15 N-labelled amino acids (Algal amino acids 13 C, 15 N, Isotec, Miamisburg, US)
and 2 μM sinigrin (PhytoLab, Vestenbergsgreuth, Germany). The diluted samples were filtered (Durapore® 0.22 μm PVDF filters, Merck Millipore, Tullagreen, Ireland) and used directly for LC-MS analysis.
and 2 μM sinigrin (PhytoLab, Vestenbergsgreuth, Germany). The diluted samples were filtered (Durapore® 0.22 μm PVDF filters, Merck Millipore, Tullagreen, Ireland) and used directly for LC-MS analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!