Total protein was extracted from lung tissues or MRC-5 cells using RIPA lysis buffer (KeyGEN, China) containing proteinase and phosphatase inhibitor. The protein concentration was measured with the BCA protein assay kit (Solarbio, USA). Aliquots of 20-40 µg proteins were separated with 8%, 12%, or 15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (Millipore, USA). After blocking with 5% skim milk (Biofroxx, Germany) or bovine albumin (Solarbio, USA), membranes were incubated with specific primary antibodies overnight at 4℃, then incubated with secondary antibodies at RT for 1.5 hours. Finally, an enhanced chemiluminescence detection kit (KeyGEN, China) was used to detect proteins and the ImageJ software was used for the grayscale analysis of protein bands. The antibodies used for western blotting are also listed in Table S1 .
Bovine albumin
Manufactured by Solarbio
Sourced in China
Bovine albumin is a protein derived from bovine serum. It is commonly used in cell culture and biomedical research applications as a supplement or stabilizer.
Automatically generated - may contain errors
Lab products found in correlation
Skim milk, by neoFroxx (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Enhanced chemiluminescence detection kit, by Keygen Biotech (1 mentions)
Bca protein assay kit, by Solarbio (1 mentions)
Ripa lysis buffer, by Keygen Biotech (1 mentions)
Penicillin streptomycin, by Cytiva (1 mentions)
Dmem f12, by Cytiva (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Trypsin edta, by Cytiva (1 mentions)
Phosphate buffered saline (pbs), by Cytiva (1 mentions)
Dmem high glucose, by Cytiva (1 mentions)
L glutamine, by Cytiva (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Meilun (1 mentions)
Chloral hydrate, by Solarbio (1 mentions)
Mannose, by Yuanye Bio-Technology (1 mentions)
Arabinose, by Yuanye Bio-Technology (1 mentions)
Galactose, by Yuanye Bio-Technology (1 mentions)
Galacturonic acid, by Yuanye Bio-Technology (1 mentions)
Rhamnose, by Yuanye Bio-Technology (1 mentions)
5 protocols using bovine albumin
1
Western Blot Analysis of Protein Expression
2
Endothelial Cell Culture Protocol
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DMEM/F12, DMEM/high glucose, phosphate-buffered saline (PBS), penicillin–streptomycin, l -glutamine and trypsin/EDTA were purchased from HyClone Laboratories Inc. (UT, USA). The culture medium of ECs was purchased from iCell Bioscience Inc. (Shanghai, China). Fetal bovine serum (FBS) was purchased from SeraPro Inc. (Beijing, China). Rabbit anti-CD31 antibody was purchased from Bioss & Technology Co., Ltd. (Beijing, China). Donkey anti-mouse antibody was purchased from Abcam Inc. (Shanghai, China). The DAPI was purchased from Meilun Biotechnology Co., Ltd. (Dalian, China). Chloral hydrate and bovine albumin (BSA) were purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). Triton X-100 and ascorbic acid were purchased from Sigma-Aldrich Co., Ltd. (MO, USA).
3
Synthesis and Characterization of 2D Nb2AlC
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Bulk Nb2AlC powder, hydrofluoric acid (HF), dimethyl sulfoxide (DMSO), N,N-dimethylformamide (DMF), and n-octadecyl mercaptan were purchased from Aladdin Reagent Co. Ltd. Biologicals including influenza A virus (Flu A), influenza B virus (Flu B), M. pneumonia (P1), chlamydia pneumoniae (CPN), immunoglobulin (IgG), prostate-specific antigen (PSA), bovine albumin (BSA), and SARS-CoV-2 and SARS-CoV-2-N58 aptamer sequence: 5′-GCT GGA TGT CAC CGG ATT GTC GGA CAT CGG ATT GTC TGA GTC ATA TGA CAC ATC CAG C-3′ were ordered from Solarbio Life Sciences Co. Ltd. Additionally, human serum was obtained from the First Affiliated Hospital of Zhengzhou University after informed consent of the patient. This research protocol was in accordance with the ethical standards of the 1964 Declaration of Helsinki and its subsequent revisions and was approved by the ethics committee of the First Affiliated Hospital of Zhengzhou University.
4
Pumpkin Peel Chromium Extraction and Evaluation
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Fresh pumpkin peel was obtained from Laiyang Mengyu Co., Ltd. (Yantai, China). CrCl3·6H2O (analytical grade) was obtained by Tianjin Kaitong chemical reagent Co., Ltd. (Shanghai, China). Trifluoroacetic acid (TFA) and insulin were provided by Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA). α-Glucosidase, 4-nitrophenyl-α-D-glucopyranoside (PNPG), penicillin-streptomycin, and albumin bovine were purchased from Beijing Solarbio Technology Co., Ltd. (Beijing, China). Acarbose and monosaccharide standards (rhamnose, arabinose, galactose, glucose, mannose, xylose, fructose, galacturonic acid, and glucuronic acid) were obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Sodium acetate, anhydrous (electrochemical grade), and SS254-500 sodium hydroxide solution were purchased from Thermo Fisher Scientific (Sunnyrale, CA, USA). High-glucose DMEM medium was obtained from Wuhan Servicebio Technology Co., Ltd. (Wuhan, China). Fetal bovine serum was purchased from Zhejiang Tianhang Biotechnology Co., Ltd. (Huzhou, China). Trivalent chromium standard solution was supplied by Beijing Century Aoke Biotechnology Co. Ltd. (Beijing, China). All other chemicals and reagents used were of analytical grade.
5
Glucosinolates Extraction and Analysis
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Coomassie brilliant blue G-250 and albumin bovine were purchased from Solarbio (Beijing, China). Anthrone and rutin trihydrate were purchased from Sinophaem (Beijing, China). Potassium nitrate and L-ascorbic acid were purchased from GCRF (Guangzhou, China). Gallic acid monohydrate was purchased from DaMao (Tianjin, China), 2,2-diphenyl-1-picrylhydrazyl was purchased from TCI (Shanghai, China), and 2,3,5-triphenyl tetrazolium chloride was purchased from Hefei Bomei (Hefei, China). Chemicals and reagents used for GSLs extraction and analysis were of liquid chromatography grade, unless otherwise stated. Sinigrin standard was purchased from Sigma-Aldrich (St. Louis, MO, USA). Sulfatase was purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). The water used was obtained from the RO/BIO Water System (18.2 MW cm, EPED Corporation, Nanjing, China).
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