Nim 811

7-day old C57BL/6 mice were used in accordance with the University of Maryland, Baltimore Institutional Animal Care and Use Committee. A streptomycin resistant derivative of the Tohama I "wildtype" B. pertussis strain was grown on Bordet-Gengou (BG) agar plates supplemented with defibrinated sheep blood (10%, Lampire Biological Products, Pipersville, Pennsylvania, USA) and 200 µg/ml streptomycin (Sigma-Aldrich, Merck). Animals were challenged via aerosol administered by a nebulizer system (Pari Vios) for twenty minutes. Lungs were excised post-euthanasia, homogenized mechanically (Omni International, Tulsa, Oklahoma, USA) and bacterial burden determined by plating on BG agar. Animals were intranasally administered vehicle (Cremophor:Ethanol:Saline 10:1:89), CsA (25 mg/kg, Sigma-Aldrich, Merck) or NIM811 (25 mg/kg, MedChemExpress, Monmouth, New Jersey, USA). To measure leukocytosis blood was harvested 7 days post-infection by cardiac puncture into ethylenediaminetetraacetic acid (EDTA) containing 1.7mL tubes (Fisher Scientific) and treated with ammonium-chloride-potassium (ACK) lysis buffer to lyse red blood cells. White blood cells were then counted using a hemocytometer.