ARV-825, dBET1, (+)-JQ1, OTX015 and pomalidomide were purchased from Cayman. MZ1, THAL SNS 032, dTAG-13 and MG132 were purchased from Tocris Bioscience. Thalidomide was purchased from Sigma. All compounds were dissolved with DMSO (Merck Millipore).
Dbet1
Manufactured by Cayman Chemical
Sourced in United States
DBET1 is a laboratory instrument designed for the analysis and detection of chemical compounds. It utilizes a combination of chromatographic and spectroscopic techniques to provide precise and accurate measurements. The core function of DBET1 is to facilitate the identification and quantification of various substances in a sample.
Automatically generated - may contain errors
Lab products found in correlation
Thalidomide, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Pomalidomide, by Cayman Chemical (1 mentions)
Mg132, by Bio-Techne (1 mentions)
Dtag 13, by Bio-Techne (1 mentions)
Otx015, by Cayman Chemical (1 mentions)
Thal sns 032, by Bio-Techne (1 mentions)
Streptomycin sulfate, by Merck Group (1 mentions)
Hek293, by American Type Culture Collection (1 mentions)
Arpe 19, by American Type Culture Collection (1 mentions)
Gt1 7 cells, by Merck Group (1 mentions)
Vh032amine, by MedChemExpress (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Vh 298, by MedChemExpress (1 mentions)
Tween 20, by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Mg132, by Merck Group (1 mentions)
4 protocols using dbet1
1
Diverse Small Molecule Compounds
2
Boron Cluster Compound Evaluation in Cell Lines
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Boron clusters (as sodium salts) were from Katchem, streptomycin sulfate and kanamycin A monosulfate both from Sigma, MMAF from Carbosynth and dBET1 from Cayman Chemicals. Peptides (WR7 and WK7) were custom-made by Biosyntan in >98% purity as confirmed by HPLC and MS. TAMRA-R8 was synthesized by solid-phase peptide synthesis, as reported51 . HeLa, HEK293, ARPE-19 and A549 cells were obtained from ATCC, and GT1-7 cells were obtained from Millipore. HeLa, HEK293, GT1-7 and A549 cells were maintained in DMEM, and ARPE-19 in DMEM/F-12, in all cases supplemented with 10% FBS and 1% penicillin–streptomycin–glutamine mix, at 37 °C, 5% CO2 and 95% humidity.
3
Affinity Reagent Procurement for Biochemical Assays
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The Tb-anti-GST antibody, 1,4-dithiothreitol
(DTT, 1 M), Tris (1 M, pH 7.5), and DMSO were purchased from Fisher
Scientific (Pittsburgh, PA). HEPES (1 M, pH 7.4) was purchased from
Teknova, Inc. (Hollister, CA). Triton X-100, Tween-20, and bovine
serum albumin were purchased from Sigma-Aldrich (St. Louis, MO). VH-298,
VH032-amine, Me-VH032-amine, and VH032-PEG4-NH2 were purchased
from MedChemExpress, LLC (Monmouth Junction, NJ). BOC-VH032 was purchased
from LabNetwork (Cambridge, MA). VH032 phenol and thalidomide-4′-O-acetamido-alkylC4-amine were purchased from Bio-Techne
Corporation (Minneapolis, MN). MZ1, dBET1, and (+)-JQ1 were purchased
from Cayman Chemical (Ann Arbor, MI). Echo 384-well low dead volume
compound plates were purchased from Labcyte, Inc. (San Jose, CA).
384-well, black low-volume assay plates were purchased from Corning
Incorporated Life Sciences (Tewksbury, MA).
(DTT, 1 M), Tris (1 M, pH 7.5), and DMSO were purchased from Fisher
Scientific (Pittsburgh, PA). HEPES (1 M, pH 7.4) was purchased from
Teknova, Inc. (Hollister, CA). Triton X-100, Tween-20, and bovine
serum albumin were purchased from Sigma-Aldrich (St. Louis, MO). VH-298,
VH032-amine, Me-VH032-amine, and VH032-PEG4-NH2 were purchased
from MedChemExpress, LLC (Monmouth Junction, NJ). BOC-VH032 was purchased
from LabNetwork (Cambridge, MA). VH032 phenol and thalidomide-4′-O-acetamido-alkylC4-amine were purchased from Bio-Techne
Corporation (Minneapolis, MN). MZ1, dBET1, and (+)-JQ1 were purchased
from Cayman Chemical (Ann Arbor, MI). Echo 384-well low dead volume
compound plates were purchased from Labcyte, Inc. (San Jose, CA).
384-well, black low-volume assay plates were purchased from Corning
Incorporated Life Sciences (Tewksbury, MA).
4
Compound Preparation and Dilution
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JQ1 (Sigma Aldrich), dBET1 (Cayman Chemicals), and MZ1 (Tocris Bioscience) were prepared as stock 2 mM solutions in 100% dimethyl sulfoxide (DMSO; Sigma Aldrich, USA) and aliquots were stored at −20 °C. Additional dilution steps were made with phosphate buffered saline (PBS) or culture medium. Thalidomide and MG132 (both Sigma Aldrich) were prepared as 100 mM (Thalidomide) and 10 mM (MG132) stock solution with DMSO, respectively.
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