Phospho p38 thr180 thr182

DT-13 was purchased from Chengdu Push Bio-technology Co., Ltd. (Chengdu, China). MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-dipheny-2-H-tetrazoliumbromide] was obtained from Amresco (Solon, OH, United States). Adriamycin (ADR) and z-VAD-FMK were from Selleck (London, ON, Canada). Hoechst 33342 and propidium iodide (PI) were purchased from Sigma-Aldrich (St. Louis, MO, United States). Antibodies against Akt, phospho-Akt (T308), phospho-PDK1 (Ser241), phospho-mTOR (Ser2448), phospho-p70S6k (Thr389), PI3K-p110α, PI3K-p110β, PARP, phospho-p38 (Thr180/Thr182), phospho-ERK1/2 (Thr202/Thr204), phospho-JNK (Thr183/Thr185), Cytc, caspase-3, caspase-8, caspase-9,Integrinβ1, phospho-Integrinβ1 and β-actin were obtained from Cell Signaling Technology (Danvers, MA, United States). FITC Annexin-V apoptosis detection kit was obtained from BD Bioscience (San Jose, CA, United States). Mitochondrial membrane potential detection kit, mitochondrial isolation kit and 2′-7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) were purchased from Beyontime Biotech (Nantong, China).

WST-8 assay kit was purchased from Dojindo Laboratories (Kumamoto, Japan). FITC Annexin V Apoptosis Detection Kit, and antibodies against phospho-Rb (S780), p27, as well as cyclin D1 were obtained from BD Biosciences (San Jose, CA, USA). Antibodies against phospho-GSK-3β (Ser9), Akt, phospho-Akt (Ser473), phospho-PDK1 (Ser241), phospho-mTOR (Ser2448), phospho-p70S6K (Thr389), PI3K-p110α, PARP, LC3B, SQSTM1/p62, Atg5, phospho-p38 (Thr180/Thr182), phospho-ERK1/2 (Thr202/Thr204) and β-actin were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibody against Lamin B, and 4′, 6-diamidino-2-phenylindole (DAPI) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Monodansylcadaverine (MDC), Propidium Iodide (PI), and 2′,7′-Dichlorofluorescein diacetate (DCFH-DA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). ptfLC3 was a gift from Tamotsu Yoshimori (Addgene plasmid #21074, Cambridge, MA, USA). Lipofectamine 2000 was obtained from Life Technologies (Carlsbad, CA, USA).