Methoxy polyethylene glycols

Methoxy polyethylene glycols were purchased from Sigma Aldrich (USA). MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide), succinic anhydride (SA), N, N dimethyl formamide (DMF), 1-ethyl-3-[3-(dimethyl amino) propyl] carbodiimide (EDC), and N-hydroxysuccinimide (NHS) were supplied by Energy Chemical Co., Ltd. (Shanghai, China). Diethyl ether and dichloromethane were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Fetal bovine serum (FBS), Dulbecco’s modified Eagle medium (DMEM), trypsin, and phosphate buffer saline (PBS) were all supplied by Thermo Fisher Scientific Co., Ltd. (USA). LDH, creatine kinase (CK), and MDA assay kits were provided by Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Hoechst 33258 staining kit and reactive oxygen species (ROS) assay kits were supplied by Beyotime biotechnology (Jiangsu, China).

All the chemicals were analytical grade and used as received without any further purifications. 4-hydroxybenzaldehyde, Methoxypolyethylene glycols, 2-(Dodecyl- thiocarbonothioylthio)-2-methylpropionic acid (DDMAT), 11-bromoundecan-1-ol, Azo-bisisobutyronitrile (AIBN), Methacryloyl chloride, 4-butylphenylethylketone, N,N-Dimethylformamide (DMF), were all purchased from Sigma-Aldrich and Alfa-Aesar. Anisole was procured from Sigma-Aldrich with extra-dry grade purification.