Random-primed cDNA synthesis was performed at 37°C starting with 0.3 μg of RNA, using the High Capacity cDNA Archive kit (Applied Biosystems, Foster City, CA). Gene expression was measured in an ABI Prism 7900HT Real Time PCR system using TaqMan FAM labeled specific probes (Applied Biosystems, , Foster City, CA [see Additional file
1 ]). Results were normalized to the housekeeping TATA-binding protein (Tbp) gene.
Taqman fam labeled specific probes
Manufactured by Thermo Fisher Scientific
TaqMan FAM labeled specific probes are oligonucleotide sequences designed for use in real-time PCR applications. They are labeled with the fluorescent dye FAM (6-carboxyfluorescein) and are specific to target sequences, allowing for the detection and quantification of target DNA or RNA.
Automatically generated - may contain errors
2 protocols using taqman fam labeled specific probes
1
Quantitative RT-PCR Expression Analysis
2
Random-primed cDNA Synthesis and RT-qPCR
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Random-primed cDNA synthesis was performed at 37°C starting with 0.3 μ g of RNA, using the High Capacity cDNA Archive kit (Applied Biosystems). Gene expression was measured in an ABI Prism 7900HT Real Time PCR system using TaqMan FAM-labeled specific probes (Applied Biosystems). A list of the probes used is presented in Supplementary Table 1 (S1 ). Results were normalized to TATA-binding protein (Tbp) expression.
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