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2 protocols using anti cd11b pe

1

Comprehensive Antibody-based Proteomics Profiling

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Antibodies used were anti-FASN (3180; Cell Signaling, Switzerland), anti-LC3B (WB: NB600-1384, Novus biological, Switzerland; IF: 3868; Cell Signaling, Switzerland) anti-LAMP1 (14-1079-80; Thermofisher, Switzerland), anti-p62 (HPA003196; Sigma-Aldrich, Switzerland), anti-TFEB (4240; Cell Signaling, Switzerland), anti-p-TFEB (Ser 211) (37681S, Cell Signaling, Switzerland), anti-p-AKT (Ser473) (4060S, Cell Signaling, Switzerland) anti-PTEN (9552, Cell Signaling, Switzerland), anti-ULK1 (4776; Cell Signaling, Switzerland), anti-p-ULK1 (Ser757, equivalent to Ser758 of human ULK1) (6888; Cell Signaling, Switzerland), anti-ATG13 (6940; Cell Signaling, Switzerland), anti-pATG13 (Ser318) (600-401-C49; Rockland, Switzerland), anti p-mTOR (Ser2448) (5536; Cell Signaling, Switzerland), p4E-BP1 (Thr37/46) (2855; Cell Signaling, Switzerland), anti-α-tubulin (3873; Cell Signaling, Switzerland), anti-cleaved PARP (9541; Cell Signaling, Switzerland), anti yH2AX (2577; Cell Signaling, Switzerland) and anti-CD11b-PE (R0841; Dako, Switzerland).
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2

Autophagy Regulatory Pathway Analysis

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Antibodies used were anti-FASN (3180; Cell Signaling, Switzerland), anti-LC3B (WB: NB600-1384, Novus biological, Switzerland; IF: 3868; Cell Signaling, Switzerland) anti-LAMP1 (14-1079-80; Thermofisher, Switzerland), anti-p62 (HPA003196 ; Sigma-Aldrich, Switzerland), anti-TFEB (4240; Cell Signaling, Switzerland) anti-ULK1(4776; Cell Signaling, Switzerland), anti p-ULK1 (Ser757) (6888; Cell Signaling, Switzerland), anti-ATG13 (6940 ; Cell Signaling, Switzerland), anti-pATG13 (Ser318) (600-401-C49; Rockland, Switzerland), anti p-mTOR (Ser2448) (5536; Cell Signaling, Switzerland), p4E-BP1 (Thr37/46) (2855; Cell Signaling, Switzerland), anti-α-tubulin (3873; Cell Signaling, Switzerland), anti-cleaved PARP (9541; Cell Signaling, Switzerland), anti yH2AX (2577; Cell Signaling, Switzerland) and anti-CD11b-PE (R0841; Dako, Switzerland).
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