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3 protocols using furfural

1

Nicotine Degradation by A. tumefaciens S33

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A. tumefaciens strain S33 was deposited with accession number CCTCC AB 2016054 at the China Type Culture Collection (CCTCC). Nicotine (1 g liter−1) was used as the sole source of carbon and nitrogen to grow strain S33 at 30°C. E. coli strain DH5α and E. coli strain BL21(DE3) were cultured in LB medium at 37°C. NAD+ and NADP+ were purchased from Sigma-Aldrich (Shanghai, China), 6-hydroxynicotine and SAP were purchased from Toronto Research Chemicals, Inc. (Toronto, Canada), and benzaldehyde, furfural, and acetaldehyde were purchased from Macklin (Shanghai, China). Carbenicillin (25 mg liter−1) and kanamycin (50 mg liter−1) were required to culture the E. coli cells.
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2

Quantification of Carbonyl Compounds

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Glyoxal (40% solution in water, high-performance LC [HPLC] grade), methylGlyoxal (40% solution in water, HPLC grade) and ethanol (99.9%, HPLC grade) were purchased from Sigma-Aldrich (Shanghai, China). Water was purified using a Milli-Q purification system (Millipore, Bedford, MA). Methanol (99.9%, LC grade), acetonitrile (ACN; 99.90%, LC grade), formic acid (99%, LC grade), furfural, 5-hydroxymethylfurfural, 5-methylfurfural, 2,3-butanedione and 2,3-pentanedione were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). 3-Deoxyglucosone was purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China).
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3

Nicotine Degradation by A. tumefaciens S33

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A. tumefaciens strain S33 was deposited with accession number CCTCC AB 2016054 at the China Type Culture Collection (CCTCC). Nicotine (1 g liter−1) was used as the sole source of carbon and nitrogen to grow strain S33 at 30°C. E. coli strain DH5α and E. coli strain BL21(DE3) were cultured in LB medium at 37°C. NAD+ and NADP+ were purchased from Sigma-Aldrich (Shanghai, China), 6-hydroxynicotine and SAP were purchased from Toronto Research Chemicals, Inc. (Toronto, Canada), and benzaldehyde, furfural, and acetaldehyde were purchased from Macklin (Shanghai, China). Carbenicillin (25 mg liter−1) and kanamycin (50 mg liter−1) were required to culture the E. coli cells.
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