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Detectx urea nitrogen bun detection kit

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The DetectX® Urea Nitrogen (BUN) Detection Kit is a quantitative colorimetric assay designed to measure urea nitrogen levels in a variety of sample types. The kit provides a simple and efficient method for the determination of urea nitrogen concentrations.

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Creatinine parameter assay kit, by R&D Systems (1 mentions) Detectx serum creatinine kit, by Arbor Assays (1 mentions)

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DetectX Urea Nitrogen Detection kit (4 citations)

2 protocols using detectx urea nitrogen bun detection kit

1

Serum and Urine Biomarkers in Murine Infection

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Blood was collected by cardiac puncture (intraperitoneal infection and survival experiment) or via the vena cava inferior from mice euthanized at defined time points. Serum enzyme levels of pancreatic amylase and alanine aminotransaminase (ALT) were measured using the EuroLyser CCA 180 Vet system (QinLAB Diagnostik) according to standard methods recommended by the International Federation of Clinical Chemistry. The Mouse TREM-1 ELISA Kit (RayBiotech), DetectX® Urea Nitrogen (BUN) Detection Kit (Arbor Assays), Mouse Lipocalin-2 (NGAL) ELISA Kit (RayBiotech) and DetectX® Serum Creatinine Kit (Arbor Assays) were used to measure the respective parameters in serum of mice. Urine of mice was collected from mice euthanized 24, 48 and 72 h p.i. from moribund and uninfected mice. Either spontaneous urine was collected or gentle trans-abdominal pressure was applied onto the bladder and urine was collected using untreated glass capillary tubes. Urinary KIM-1 and creatinine levels were measured using the Mouse TIM-1 ELISA Kit (RayBiotech) and the Creatinine Parameter Assay Kit (R&D Systems), respectively. KIM-1 levels were normalized to urinary creatinine to account for differences in urinary concentration.
Dunker C., Polke M., Schulze-Richter B., Schubert K., Rudolphi S., Gressler A.E., Pawlik T., Prada Salcedo J.P., Niemiec M.J., Slesiona-Künzel S., Swidergall M., Martin R., Dandekar T, & Jacobsen I.D. (2021). Rapid proliferation due to better metabolic adaptation results in full virulence of a filament-deficient Candida albicans strain. Nature Communications, 12, 3899.
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2

Ischemia-Reperfusion Injury in Mice

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Male mice (littermates, 8 ~12 weeks old) were anesthetized via
intraperitoneal injection of ketamine (120 mg/kg) and xylazine (12 mg/kg)
and then subjected to bilateral or unilateral kidney IRI. Briefly, IRI was
performed through flank incisions by clamping the renal pedicles for
indicated times. Once the clamps were removed and restoration of blood flow
was visually observed, the wound was sutured closed. Sham-operated mice
underwent the same procedure except that the renal pedicles were not
clamped. Mice received buprenorphine SR (0.5 mg/kg) as a postoperative
analgesic. Kidneys were allowed to reperfuse for the indicated times. For
unilateral IRI, 1×1012 AAV-TELMO or a control AAV in 75
µL PBS were injected into the renal vein while the renal pedicles
were clamped. Fourteen days after reperfusion, the contralateral right
kidney was removed to evaluate the function of the left kidney. Blood urea
nitrogen (BUN) was measured using the DetectX Urea Nitrogen (BUN) Detection
kit (Arbor Assays, Ann Arbor, MI). Creatinine was measured as described
above. The mice were euthanized with an overdose of ketamine and xylazine,
and blood was collected from the retroorbital sinus. The kidneys were
harvested for histological analysis and RNA extraction. All mouse procedures
were performed by approved IACUC protocols.
Morioka S., Kajioka D., Yamaoka Y., Ellison R.M., Tufan T., Werkman I.L., Tanaka S., Barron B., T Ito S., Kucenas S., Okusa M.D, & Ravichandran K.S. (2022). Chimeric efferocytic receptors improve apoptotic cell clearance and alleviate inflammation. Cell, 185(26), 4887-4903.e17.
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