6460 triple quadrupole qqq mass spectrometer

An Agilent 1290 series UHPLC system coupled to a 6460 Triple Quadrupole (QqQ) mass spectrometer (both Agilent Technologies, Waldbronn, Germany) was used to analyze the samples. Precursor and product ion selection as well as the optimization of collision energies were performed with flow injection of single-analyte solutions. UHPLC separations were performed in a reversed-phase C18 analytical column of 50 mm × 2.1 mm and 1.8 μm particle size (ZORBAX RRHD Eclipse Plus C18) by Agilent Technologies (Waldbronn, Germany).
The chromatographic solvents were water 0.1% formic acid solution (eluent A) and acetonitrile 0.1% formic acid (eluent B). The gradient program was as follows: 0.0 min, 10% B; 2.4 min, 42% B; 6.0 min, 51% B; 6.2 min, 95% B; 7.0 min, 10% B. A subsequent re-equilibration time (5 min) should be performed before next injection. The constant flow rate was 0.3 mL/min while the injection volume was 2 μL. Moreover, the column temperature was maintained at 30 °C.
MS/MS analyses of mycotoxins were performed on an 6460 QqQ mass spectrometer with Agilent Jet Stream Technology under the dynamic multiple reaction monitoring (DMRM) conditions in ESI⁺. The following settings were used: nebulizer, 40 psi; drying gas temperature, 350 °C; drying gas flow, 10 L/min; capillary voltage, 4000 V.

The analyses were conducted on an HPLC-MS system with Agilent 1290 Infinity II quaternary pump, column thermostat, an autosampler and an Agilent 6460 Triple Quadrupole (QqQ) mass spectrometer (MS) with Agilent Jet Stream Technology (heated electrospray) ionization source (ESI), as reported by [22 (link)] with slight modifications. A Zorbax Eclipse Plus C18 (3.0 × 100 mm, 1.8 μm) column was employed and 0.1% aqueous formic (A) and acetonitrile (B) were used as mobile phases. The gradient program for the analyses was: 0–2 min, 10% B; 2–13 min, 10–25% B; 13–32 min, 25–75% B; 32–35 min, 75–100% B, 35–37 min, 100% B and 37–38 min, 100–10% B; the total cycle time was 43 min; flow rate: 0.5 mL/min. The ESI and MS parameters: drying gas temperature 320 °C, drying gas flow 9 L/min, nebulizer gas pressure 45 psi, sheath gas flow 12 L/min, sheath gas temperature 400 °C, capillary voltage 3000 V and nozzle voltage 0 V. Neutral loss scan mode was performed with the neutral loss of 46 in the m/z range from 50 to 600, collision energy 8 V and fragmentor 90 V. The data was processed using the Agilent MassHunter Qualitative Analysis Navigator B.08.00 software. The quantification of the detected compounds was performed in the NLS mode employing the single-point external calibration method.