Norephedrine

EH (JP16 grade) originally produced from E. sinica was purchased from Uchida Wakanyaku Co., Ltd. The authentic EAs used were: ephedrine, purchased from Dainippon Pharma Co., Ltd.; methylephedrine and pseudoephedrine, from Alps Pharmaceutical Ind., Co., Ltd.; and norephedrine from Tokyo Chemical Industry Co. Ltd. 6-Methoxykynurenic acid was purchased from Chemicia Scientific, LLC. trans-Cinnamic acid was purchased from Wako Co., while herbacetin 7-O-neohesperidoside and herbacetin 7-O-glucoside were isolated from EH [15 (link)]. Herbacetin and apigenin were purchased from ChromaDex Co. and Wako Co., respectively.

Ephedrine was purchased from Dainippon Pharma Co., Ltd. (Tokyo, Japan). Methylephedrine and pseudoephedrine were purchased from Alps Pharmaceutical Ind. Co. Ltd. (Gifu, Japan). Norephedrine was purchased from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). 6-Methoxykynurenic acid was purchased from Chemicia Scientific, LLC (San Diego, CA, USA). trans-Cinnamic acid was purchased from Wako Co. (Tokyo, Japan). 6-Hydroxykynurenic acid was synthesized from 6-methoxykynurenic acid [14 ]. Syringin, kaempferol 3-O-rhamnoside 7-O-glucoside, and isovitexin 2″-O-rhamnoside were isolated from Ephedra Herb [12 (link)]. The methanol and water used for liquid chromatography coupled with photodiode array detection (LC-PDA) analysis were LC grade.
Recombinant human HGF (purity ≥98 % by SDS-PAGE and HPLC analysis) was purchased from PeproTech Inc. (Rocky Hill, NJ, USA). SU11274 (purity ≥98 % by HPLC analysis) was purchased from Sigma-Aldrich (St Louis, MO, USA). The antibodies (Abs) used were as follows: anti-p-Met (Tyr1234/1235) monoclonal Ab (mAb) (CST#3077), anti-Met mAb (CST#8198), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPD) mAb (CST#2118), and horseradish peroxidase (HRP)-labeled anti-rabbit IgG Ab (CST#7074); all were obtained from Cell Signaling Technology Japan, K.K. (Tokyo, Japan).