Lna scr

miR-34a^fl/fl mice, albumin-Cre (Alb-Cre) mice, and C57BL/6J mice were purchased from the Jackson Laboratory (Bar Harbor, Maine, USA) [4 (link)]. miR-34a^fl/fl mice were crossed with Alb-Cre mice to generate germline hepatocyte-specific miR-34a^−/− (miR-34a^gHep−/−) mice and control littermates (miR-34a^fl/fl mice). AAV8-TBG-Null or AAV8-TBG-Cre was i.v. injected into the miR-34a^fl/fl mice to generate control mice (miR-34a^fl/fl mice) or adult-onset hepatocyte-specific miR-34a^−/− (miR-34a^Hep−/−) mice, respectively. Locked nucleic acids (LNA) against scramble sequences (LNA-Scr) or miR-34a (LNA-miR-34a) were synthesized by Qiagen and i.p. injected into the mice at a dosage of 10 mg/kg/week. All of the mice were housed in a temperature- and humidity-controlled room with a 12-h light/12-h dark cycle under pathogen-free conditions. The high-fat/cholesterol/fructose (HFCF) diet contained 40% fat/0.2% cholesterol (AIN-76A Western diet from TestDiet) and 4.2% fructose (in drinking water). Unless otherwise stated, 2-month-old male mice were used and fed an HFCF diet for 16 weeks. The mice were fasted for 5–6 h during the light cycle prior to euthanasia. All the of animal experiments were approved by the Institutional Animal Care and Use Committee at Northeast Ohio Medical University (NEOMED).