Sixty nulliparous female Wistar rats weighing 180 to 200 g were housed in a temperature-controlled animal facility with a reversed 12:12-hour light/dark cycle. All rats were fed normal rat chow. All animals and experimental procedures were approved by the Animal Care and Use Committee at the China Medical University (Permit#: TZ2020009), which complies with the National Institute of Health Guide for the Care and Use of Laboratory Animals. The day of visualization of spermatozoa in vaginal smears was taken as embryonic (E) day 0. The day of birth was designated as postnatal (P) day 0. The pregnant rats were divided randomly into euthyroid (n=30 dams) and hypothyroid (n=30 dams) groups. To induce developmental hypothyroidism, a thyrotoxicant (2-mercapto-1-methylimidazole [MMI]; 0.025% w/v; 301507, Sigma, St. Louis, MO, USA), was given to pregnant rats in their drinking water from E6 and continued until the rats were sacrificed.
2 mercapto 1 methylimidazole mmi
Manufactured by Merck Group
Sourced in United States
2-mercapto-1-methylimidazole [MMI] is a heterocyclic organic compound used as a laboratory reagent. It contains a 1-methylimidazole core structure with a thiol (mercapto) group attached at the 2-position.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using 2 mercapto 1 methylimidazole mmi
1
Gestational Hypothyroidism in Wistar Rats
2
Thyroid Hormone Synthesis Modulation
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Commercial sources for reagents used for supplemental experiments were as follows:
3,3′,5-Triiodo-L-thyronine (T3; Sigma-Aldrich), 2-Mercapto-1-methylimidazole (MMI; Sigma-Aldrich), Sodium perchlorate monohydrate (PM; Sigma-Aldrich).
3,3′,5-Triiodo-L-thyronine (T3; Sigma-Aldrich), 2-Mercapto-1-methylimidazole (MMI; Sigma-Aldrich), Sodium perchlorate monohydrate (PM; Sigma-Aldrich).
3
Thyroid Hormone Regulation of Xenograft Tumors
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HuH7 xenograft tumours were established by s.c. injection of 3.5!10 6 HuH7 cells in 100 ml PBS into the flank region. Three days later, animals received drinking water supplemented with 0.02% (w/v) 2-mercapto-1-methylimidazole (MMI; Sigma-Aldrich), 1% (w/v) sodium perchlorate (Sigma-Aldrich) and 0.3% (w/v) saccharin (Sigma-Aldrich) (Groba et al. 2013) to induce hypothyroidism in order to generate the same baseline thyroid hormone levels for all groups. Three weeks later, once small tumours (!3 mm) were visible, mice were randomly assigned to different groups by daily i.p. injections. The hyperthyroid group (nZ8) received 100 ng/g body weight L-T 4 (Sigma-Aldrich), the euthyroid group 20 ng/g body weight L-T 4 with (nZ8) or without (nZ7) 10 mg/g body weight tetrac (Sigma-Aldrich), hypothyroid animals (nZ8) received saline only. Eighteen days later (tumour volume
4
Investigating Cellular Signaling Pathways
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Akt 1/2 kinase inhibitor (Akti 1/2 ), wortmannin, LY294002, BAY 11-7082, 2-Phenyl-1,2-benzisoselenazol-3-(2H)-one (Ebselen), N-acetylcysteine (NAC), 6-carboxy-2 0 ,7 0 -dichlorodihydrofluorescein diacetate (carboxy-H2-DCFDA), actinomycin D, 2-mercapto-1methyl-imidazole (MMI), bovine serum albumin (BSA) and sodium iodide were purchased from Sigma Chemical Co. (St. Louis, MO).
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