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Pf670462

Manufactured by Abcam
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Sourced in Japan

PF670462 is a small molecule compound that functions as an inhibitor. It is available for research use.

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Lab products found in correlation

Mouse anti flag m2, by Merck Group (1 mentions) Mouse anti α tubulin, by Merck Group (1 mentions) Pf 06447475, by Merck Group (1 mentions) Ic261, by Merck Group (1 mentions) Mouse anti vinculin, by Merck Group (1 mentions)

2 protocols using pf670462

1

Circadian Clock Modulation in Mice

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We used 4-[1-Cyclohexyl-4-(4-fluorophenyl)-1H-imidazol-5-yl]-2-pyrimidinamine dihydrochloride (PF670462, Abcam, Tokyo, Japan) to inhibit Clock/Bmal1 activity in mice. PF670462 is a potent, selective Casein Kinase 1ε (CK1ε) and CK1δ inhibitor and PER2 activator. We subcutaneously administered PF670462 (50 mg/kg/day) suspended in saline on each day of the experiment 14 (link).
Hiramoto K., Yamate Y., Kasahara E, & Sato E.F. (2018). An Inhibitor of Casein Kinase 1ε/δ (PF670462) Prevents the Deterioration of Dextran Sodium Sulfate-induced Ulcerative Colitis Caused by UVB Eye Irradiation. International Journal of Biological Sciences, 14(9), 992-999.
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2

LRRK2 Kinase Inhibitors and Constructs

Cited 1 time
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LRRK2 kinase inhibitors CZC-25146 and PF-06447475 and casein kinase 1 inhibitor IC261 were purchased from Sigma-Aldrich. Casein kinase 1 inhibitor PF-670462 was purchased from Abcam. LRRK2 kinase inhibitor MLi-2 was kindly provided by Dr. D. Alessi (Division of Signal Transduction Therapy, University of Dundee). pCHMWS_3Flag_LRRK2_Ires_Hygro constructs of pathogenic (R1441C/G, Y1699C, and I2020T) LRRK2 variants were cloned as described in [42 (link)]. The pCHMWS_3Flag_LRRK2_Ires_Hygro constructs encoding truncated variants PLRCKW and APLRCKW, as well as the LRRK2 S908A/S910A/S935A/S955A/S973A/S976A or S908E/S910E/S935E/S955E/S973E/S976E were generated using gBlock® Gene Fragments (IDT) and as described in [42 (link)]. The following antibodies were used: mouse anti-FlagM2 (Sigma-Aldrich, F1804), mouse anti-vinculin (Sigma-Aldrich, V9131), mouse anti-α-tubulin (Sigma-Aldrich, T5168), rabbit anti-LRRK2 P-S935 (Abcam, ab133450), rabbit anti-LRRK2 P-S1292 (Abcam, ab203181), rabbit anti-LRRK2 MJFF-2 antibody (Abcam, ab133474), mouse anti-LRRK2/Dardarin, N-terminus N138/6 (Neuromab 75-188), mouse anti-LRRK2/Dardarin, C-terminus N241A/34 (Neuromab 75-253), mouse anti-LRRK2 MC.028.83.76.242 (ab130277).
De Wit T., Baekelandt V, & Lobbestael E. (2018). Inhibition of LRRK2 or Casein Kinase 1 Results in LRRK2 Protein Destabilization. Molecular Neurobiology, 56(8), 5273-5286.
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