Bq 123

BQ-123 (HY-12378, MedChemExpress) and BQ-788 (HY-15894, MedChemExpress) were dissolved in dimethyl sulfoxide (DMSO) according to the manufacturer’s instructions and then diluted in PBS to a final bath concentration of 1% DMSO. After 6 weeks of C57 mouse infection with cercariae, Vehicle (1% DMSO), BQ-123, BQ-788, and BQ-123 plus BQ-788 were intraperitoneally (i.p.) administered to mice at doses of 50 μg/kg three times a week for 6 weeks. Then, mice were harvested for further analysis.

BIM, bovine serum albumin (BSA), BQ-788, 4′,6-diamidino-2-phenylindole (DAPI), ET-1, Ficoll^® PM 400 (Ficoll), LPS from Escherichia coli 0111:B4, TAK-242, and WP9QY were purchased from Merck (Darmstadt, Germany). H398 and TNF-α were obtained from Enzo Life Science (Farmingdale, NY, USA) and FUJIFILM Wako Chemicals (Tokyo, Japan), respectively. TAPI-0 and anti-TLR4 antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). BQ-123 and PA were obtained from MedChemExpress (Monmouth Junction, NJ, USA) and AG Scientific (San Diego, CA, USA), respectively. L-NMMA was purchased from Dojindo (Kumamoto, Japan). NBD-CSA was synthesized following our previous report by Tajima et al. [7 (link)]. All other reagents were commercially obtained.