Levels of Aβ40 and Aβ42, corresponding to total Aβ load, was measured in the formic acid (FA) treated brain homogenates, as previously described [25 (link)]. In brief, 96-well plates were coated overnight with 100 ng/well of rabbit polyclonal anti-Aβ40 or anti-Aβ42 (custom made by Agrisera) and blocked with 1% BSA in PBS. FA extracts from frontal cortex were neutralized with 2 M tris and diluted 2000× for Aβ40 and 200× for Aβ42 analysis, then incubated overnight, followed by detection with biotinylated mAb1C3 (0.5μg/ml) [26 (link)] and streptavidin-HRP (Mabtech AB). Signals were developed with K Blue Aqueous TMB substrate (Neogen Corp.) and read with a spectrophotometer at 450 nm. All dilutions were made in ELISA incubation buffer (PBS with 0.1% BSA, 0.05% Tween20 and 0.15% Kathon).
Anti aβ40
Manufactured by Agrisera
Sourced in Sweden
Anti-Aβ40 is a laboratory product that can be used to detect the presence of amyloid-beta 40 (Aβ40) peptide, a major component of amyloid plaques associated with Alzheimer's disease. The product is intended for research purposes only and its core function is to provide a tool for the identification and quantification of Aβ40 in biological samples.
Automatically generated - may contain errors
2 protocols using anti aβ40
1
Quantification of Amyloid-beta Peptides
2
Immunohistochemical Analysis of Alzheimer's Pathology
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Right hemispheres from fresh frozen mouse brains were sectioned at 20 µm. Next, the sections were fixed with 4% paraformaldehyde and treated with pre-heated citrate buffer, pH 6.3, for 30 min followed by 70% formic acid for 5 min. Aβ was visualized with anti-Aβ40 (Agrisera, Umeå, Sweden), anti-Aβ42 1:1000 (700254, Thermo Fisher Scientific, USA), mAb158 or 3D6 1:1000 (in-house expression); activated astrocytes with anti-GFAP 1:200 (Abcam, Cambridge, UK); and microglia with antibodies against Iba1 (1:200) (Wako chemicals, Richmond, VA) and TREM2 1:200 (AF1729; R&D, Abingdon, UK). For colorimetric staining the Vector NovaRED™ horse radish peroxidase (HRP) substrate kit (Vector Laboratories, Burlingame, CA) was used for detection while for fluorescent staining Alexa secondary antibodies were used (Thermo Fisher Scientific, USA). For thioflavin S (ThS) staining, sections were pretreated in 95% and 70% ethanol (3 min in each), and quickly rinsed in water before they were incubated in 0.1% ThS for 10 min. Finally, the sections were briefly washed in 80% ethanol and water, dehydrated in ethanol, cleared in xylene and mounted with DPX.
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