Animal procedures and maintenance were conducted in accordance with the University of Wisconsin-Madison School of Medicine and Public Health IACUC guidelines. PDXs were propagated using R2G2 mice (Envigo, Indianapolis, IN, USA), then inoculated by subcutaneous injection into the dorsal flank of female athymic nude mice (Envigo). MOC cell lines were inoculated by subcutaneous injection into the dorsal flank of 7–8 weeks old female C57BL/6N (Charles River Laboratories) or female athymic nude mice (Envigo). All cells were resuspended in PBS containing Matrigel (50% v/v, Corning Inc., Corning, NY, USA). When tumors attained a volume of ~200mm3 , as measured by a caliper, mice were randomized into groups and treatment was initiated. Inhibitor or equivalent volume of vehicle was administered by intraperitoneal injection (anti-PDL1 or IgG), oral gavage (R428, MRX2843, INCB081776), or chow (INCB081776).
R2g2 mice
Manufactured by Inotiv
Sourced in United States
The R2G2 mice are a type of laboratory mouse model. They are genetically engineered to express specific genetic modifications. The core function of the R2G2 mice is to serve as a research tool for scientific investigations.
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Lab products found in correlation
2 protocols using r2g2 mice
1
Preclinical Evaluation of Anti-Cancer Agents
2
Radiolabeled ofatumumab in R2G2 mice
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The Washington University in St. Louis Institutional Animal Care and Use Committee approved the animal studies. Eight- to 10-wk-old R2G2 mice (no. 021; Envigo) were inoculated subcutaneously with 5 × 106 Raji-Luc cells. When palpable tumors were present, 8–11 μg (4.07 kBq) of [225Ac]Ac-ofatumumab were administered intravenously and biodistribution analyzed 7 d later. The femur was measured after marrow extraction.
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