Taqman rt pcr kit

K18-hACE2 KI mice, which express hACE2 driven by the human epithelial cell cytokeratin-18 (K18) promoter, were purchased from Gempharmatech. All animal experiments were approved by the Animal Care and Use Committee of Wuhan University. For antibody prophylactic experiments, mice were grouped for injection of antibodies (10 mg/kg). At dpi 0, mice were inoculated intranasally with 250 PFU SARS-CoV-2. For antibody therapeutic effect, mice were inoculated with 250 PFU of SARS-CoV-2 by the intranasal route, and one day later, mice were injected with antibodies (20 mg/kg). Body weights were monitored daily, and the endpoint is at dpi 8 or when mice dead or when mice’s weight reached 80% of starting weight. Tissues were harvested for tissue homogenates and histology analysis. The H&E and immunofluorescence stain and imaging were carried by Wuhan Pinuofei Biological Technology company. The viral gene copies were detected by a TaqMan RT-PCR Kit (Yeason). To accurately quantify the absolute number of SARS-CoV-2 genomes, a standard curve was prepared by measuring the SARS-CoV-2 E and N gene plasmid.^{39 (link)} All animal studies were approved by the Institutional Animal Care and Use Committee at Wuhan University (WP20220044).