Il 1β d3u3e

Manufactured by Cell Signaling Technology

Sourced in United States

IL-1β (D3U3E) is an antibody that recognizes the interleukin-1 beta (IL-1β) protein. IL-1β is a pro-inflammatory cytokine that plays a key role in the body's immune response.

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Lab products found in correlation

Phospho drp1 ser616, by Cell Signaling Technology (1 mentions) Phospho nf κb p65 ser536 93h1, by Cell Signaling Technology (1 mentions) Nf κb p65, by Cell Signaling Technology (1 mentions) Apoptosis and necrosis assay kit, by Beyotime (1 mentions) Sybr green and reverse transcription kit, by Roche (1 mentions) Total superoxide dismutase assay kit with nbt, by Beyotime (1 mentions) Ros assay kit, by Beyotime (1 mentions) Ldh cytotoxicity assay kit, by Beyotime (1 mentions) Lipid peroxidation mda assay kit, by Beyotime (1 mentions)

2 protocols using il 1β d3u3e

Antibodies for Western Blot and Immunofluorescence

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All antibodies used for Western blot and immunofluorescence were described in Table 4.Table 4

The antibodies were used in Western blot and immunofluorescence

Antibody	Source	Identifier	Dilution
Sirt3	HUABIO	R1511-3	1:750
ACOD1	Abcam	ab222411	1:1000
Acetylated-lysine antibody	Cell Signaling Technology (CST)	9441	1:1500
Anti-SOD2/MnSOD (acetyl K68)	Abcam	ab137037	1:1000
Integrin beta 7	Proteintech	11,328–1-AP	1:1000
NF-κB p65	HUABIO	ET1603-12	1:1000
Phospho-NF-κB p65 (Ser536) (93H1)	Cell Signaling Technology (CST)	3033S	1:750
Phospho-DRP1 (Ser616)	Cell Signaling Technology (CST)	3455S	1:750
AMPK alpha 1	HUABIO	ET1608-40	1:1000
Phospho-AMPKα (Thr172) (D4D6D)	Cell Signaling Technology (CST)	2535S	1:750
CCL2	HUABIO	HA500267	1:1000
CCR2	HUABIO	ET1611-65	1:1000
iNOS	Abcam	ab178945	1:1500
COX2/Cyclooxygenase 2	HUABIO	ET1610-23	1:1500
NLRP3(NLRC3)	HUABIO	ET1610-93	1:1500
IL-1β (D3U3E)	Cell Signaling Technology (CST)	12703S	1:1000
Caspase-1	Adipogene	AG-20B-0042-C100	1:1000
Myeloperoxidase (MPO)	Abcam	ab208670	1:100
ASC(WB)	Adipogene	AG-25B-0006PF-C100	1:1000
ASC(IF)	Santa Cruz Biotech	Sc-514414	1:50
Tubulin	HUABIO	ET1602-4	1:1500
GAPDH	HUABIO	HA721136	1:1500

Lv L., Jiang H., Song D., Zhou X., Chen F., Ren L., Xie Y, & Zeng M. (2023). Sirt3 improves monosodium urate crystal-induced inflammation by suppressing Acod1 expression. Arthritis Research & Therapy, 25, 121.

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Quercetin Attenuates Ox-LDL-Induced Inflammation

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The following drugs and reagents were used in the present study: quercetin (C₁₅H₁₀O₇, 98% purity), was obtained from Chengdu Herbpurify (Chengdu, China). Ox-LDL was purchased from Yiyuan Biotechnology (Guangzhou, China). SYBR Green and Reverse Transcription kit were obtained from Roche (Basel, Switzerland). Primary antibody sources are as follows: antibodies against NLRP3 (D4D8T), caspase1 (D7F10), N-GSDMD (93709), cle-caspase1 (D57A2), NRF2 (D1Z9C), Heme oxygenase-1(HO-1) (E9H3A), KEAP1 (P586) and IL-1β (D3U3E) were purchased from Cell Signaling Technology (Boston, USA), antibody against β-actin and Goat anti-rabbit antibody were obtained from Merck (Darmstadt, Germany). Lipid Peroxidation MDA Assay Kit, ROS Assay Kit, Total Superoxide Dismutase Assay Kit with NBT, LDH Cytotoxicity Assay Kit and Apoptosis and Necrosis Assay Kit are obtained from Beyotime Biotechnology (Shanghai, China). Enzyme linked immunosorbent assay (ELISA) kits for IL-1β and IL-18 was obtained from Ebioscience (Wuhan, China).

Luo X., Weng X., Bao X., Bai X., Lv Y., Zhang S., Chen Y., Zhao C., Zeng M., Huang J., Xu B., Johnson T.W., White S.J., Li J., Jia H, & Yu B. (2022). A novel anti-atherosclerotic mechanism of quercetin: Competitive binding to KEAP1 via Arg483 to inhibit macrophage pyroptosis. Redox Biology, 57, 102511.

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