To characterize changes in immune cell infiltration associated with histotripsy, routine immunohistochemistry was performed on formalin-fixed, paraffin-embedded (FFPE) sections of tumor taken prior to treatment, untreated tumor sampled following histotripsy treatment, and treated tumor sampled after histotripsy treatment. Tumor-associated macrophages (TAMs) and tumor-infiltrating lymphocytes (TILs) were investigated. All immunohistochemical stains were run on a Roche Ventana Discovery Ultra Automated Stainer using a heated Tris buffer for antigen retrieval and the Ultraview Red system detection system. Tissues were stained with antibodies against IBA-1 (FujiFilm, 019-19741), CD3 (Agilent Dako, A0452), and CD79a (Santa Cruz Biotechnology, HM47). All slides were counterstained with hematoxylin.
Ventana discovery ultra automated stainer
Manufactured by Roche
The Ventana Discovery Ultra Automated Stainer is a laboratory equipment designed for the automated staining of tissue samples. It performs immunohistochemistry (IHC) and in situ hybridization (ISH) assays.
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Lab products found in correlation
2 protocols using ventana discovery ultra automated stainer
1
Histotripsy-Induced Immune Cell Changes
2
Histotripsy-Induced Immune Cell Changes
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To characterize changes in immune cell infiltration associated with histotripsy, routine immunohistochemistry was performed on formalin-fixed, paraffin-embedded (FFPE) sections of tumor taken prior to treatment, untreated tumor sampled following histotripsy treatment, and treated tumor sampled after histotripsy treatment. Tumor-associated macrophages (TAMs) and tumor-infiltrating lymphocytes (TILs) were investigated. All immunohistochemical stains were run on a Roche Ventana Discovery Ultra Automated Stainer using a heated Tris buffer for antigen retrieval and the Ultraview Red system detection system. Tissues were stained with antibodies against IBA-1 (FujiFilm, 019-19741), CD3 (Agilent Dako, A0452), and CD79a (Santa Cruz Biotechnology, HM47). All slides were counterstained with hematoxylin.
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