The human TK6 lymphoblastoid and the human AML cell lines (OCI-AML2 and MOLM-13) were obtained from Cobioer Biosciences Co., Ltd. (Nanjing, China). The cells were cultured in RPMI-1640 medium (cat. no. AH411-0272400047; Thermo Fisher Scientific, Inc.) containing penicillin-streptomycin (100 IU/ml and 100 mg/ml respectively) and 20% FBS (cat. no. 10100147; Thermo Fisher Scientific, Inc.). The cells were cultured at 37°C in an incubator with 5% CO2 (cat. no. OM-80A-II; Shanghai Huaiyu Instrument Equipment Co., Ltd). White blood cell isolation from the peripheral blood was performed using a Human Peripheral Blood Leukocyte Isolation kit (cat. no. P8670; Beijing Solarbio Science & Technology Co., Ltd.), according to the manufacturer's instructions.
Molm 13
Manufactured by Cobioer Biosciences
Sourced in China
The MOLM-13 is a laboratory equipment designed for cell culture and analysis. It is a liquid handling system capable of pipetting and dispensing small volumes of liquids with high precision and accuracy. The MOLM-13 can be used for a variety of applications, including but not limited to, sample preparation, reagent addition, and plate filling in life science research and drug discovery workflows.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using molm 13
1
Cell Culture and Blood Cell Isolation
2
FLT3 Mutant Cell Line Development
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LT-171-861 was originally designed and synthesized by China Pharmaceutical University. Sorafenib (Cat. #3009) and AC220 (Cat. #5793) were purchased from ApexBio Company (Houston, TX). The human leukemia cell lines MV4-11, MOLM13, and murine pro-B cell line BaF3 were purchased from Cobioer company (Nanjing, China). Human leukemia cell line THP-1, U937 and human marrow stromal cell line HS-5 were originally obtained from the Cell Bank of Shanghai Institute of Cell Biology (Shanghai, China). MV4-11 cells were cultured in Iscove's Modified Dulbecco's Medium (IMDM) (Grand Island, NY) containing 10% FBS (Grand Island, NY). MOLM13, THP-1, and U937 cells were cultured in RPMI 1640 medium (Grand Island, NY) with 10% FBS. Murine pre-B cell line BaF3 was cultured in RPMI 1640 (Grand Island, NY) containing 10% FBS and 3 ng/mL interleukin (IL)-3 (R&D, MN, USA). HEK‑293T and HS-5 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) (Grand Island, NY) supplemented with 10% FBS. Plasmids for construction of transformed BaF3-FLT3-wt and BaF3-FLT3-ITD were obtained from Dr. Bailiang He from The University of Hong Kong. A site-directed mutagenesis kit (Vazyme Biotech Co., Ltd, Nanjing, China) was used to generate plasmids for the construction of transformed BaF3-FLT3-ITD-F691L, BaF3-FLT3-ITD-N676D, BaF3‑FLT3-ITD-Y842C and BaF3-FLT3-D835Y.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!