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Formate dehydrogenase from candida boidinii

Manufactured by Merck Group
Sourced in United States

Formate dehydrogenase from Candida boidinii is a laboratory enzyme used for the oxidation of formate to carbon dioxide. The enzyme catalyzes the conversion of formate to carbon dioxide while reducing NAD+ to NADH.

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2 protocols using formate dehydrogenase from candida boidinii

1

Perovskite Solar Cell Material Synthesis

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Formamidinium iodide (FAI), methylammonium bromide (MABr), and TiO2 paste (30NR-D) were purchased from the Dyesol Ltd (Australia). All other chemicals including β-nicotinamide adenine dinucleotide hydrate (NAD+, ≥96.5%), α-ketoglutaric acid disodium salt dihydrate (≥98.0%), ammonium sulfate (≥99.0%), l-glutamate dehydrogenase from bovine liver (GDH, ammonium sulfate suspension), and formate dehydrogenase from Candida boidinii (FDH, lyophilized power) were purchased from the Sigma-Aldrich Corp. (USA) and used without further purification.
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2

Candida boidinii Formate Dehydrogenase Production

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Formate dehydrogenase from Candida boidinii (CAS number: 9028‐85‐7, catalog number: F8649‐50UN) was produced from Sigma‐Aldrich, USA. NADH (molar mass: 709.4, CAS number: 606‐68‐8 (anhydrous), part number: MFCD00036200), potassium dihydrogen phosphate, sodium chloride, potassium chloride, and disodium hydrogen phosphate were produced from Macklin, China. Zinc acetate and zinc nitrate hexahydrate was made from Sinopharm Chemical ReagentCo., Ltd, China. 2‐Melm was created from Xiya Reagent, China. Sodium nitrate and neutral red were produced from Tianjin Kemiou Chemical Reagent Co., Ltd., China. Imidazole‐2‐formaldehyde (2‐ICA) was produced by Shanghai Maclin Biochemical Technology Co., Ltd., China.
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