Immunoglobulin g igg antibody

Manufactured by Merck Group

Sourced in United States

Immunoglobulin G (IgG) antibody is a type of antibody protein produced by plasma B cells. It is the most abundant antibody isotype found in the blood and extracellular fluid, and plays a crucial role in the humoral immune response. IgG antibodies are capable of neutralizing toxins and viruses, as well as activating the complement system to eliminate pathogens.

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Lab products found in correlation

Magna rna binding protein immunoprecipitation kit, by Merck Group (1 mentions) Ago2 antibody, by Merck Group (1 mentions) Anti argonaute2 ago2 antibody, by Abcam (1 mentions) Rna binding protein immunoprecipitation kit, by Geneseed (1 mentions)

Close spelling variants of this product

Anti-immunoglobulin G (IgG) antibody Fluorescein-labeled goat anti-mouse immunoglobulin G (IgG) antibody Horseradish peroxidase-conjugated anti-mouse and anti-rabbit immunoglobulin G (IgG) antibody Goat anti-mouse immunoglobulin G (IgG) peroxidase-conjugated antibody Immunoglobulin G (IgG,

2 protocols using immunoglobulin g igg antibody

RIP Assay for Ago2-bound RNA

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RIP assay was carried out through Magna RNA-binding protein immunoprecipitation kit (Millipore, Billerica, MA). Briefly, cell lysate (H9c2 cells) was incubated in RIP buffer containing magnetic beads conjugating Argonaute 2 (Ago2) antibody (Millipore). Immunoglobulin G (IgG) antibody (Millipore) acted as control. Immunoprecipitated RNA was isolated, purified, and then analyzed by RT-qPCR analysis. Bio-repeats were conducted in triplicate.

Liu M., Zhang Y., Li Y., Shi T, & Yan Y. (2024). LncRNA Zfas1 boosts cell apoptosis and autophagy in myocardial injury induced by hypoxia via miR-383-5p/ATG10 axis. Heliyon, 10(3), e24578.

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Profiling CircCOL1A1 and miR-149-5p Interactions

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The RIP assay was performed with the RNA-Binding Protein Immunoprecipitation Kit (Geneseed, Guangzhou, China) following the manufacturer’s instructions. A total of 1 × 10⁷ hair follicle stem cells were seeded and grown to ∼70–80% confluence. Then, the stem cells were collected and lysed using RIP lysis buffer. Subsequently, the pooled lysates were equally divided into two parts for incubation overnight at 4°C with immunoglobulin G (IgG) antibody (Millipore, Massachusetts, United States) and anti-Argonaute-2 (Ago-2) antibody (Abcam, Cambridge, United Kingdom). Then, the immunoprecipitated RNA was extracted and purified. Finally, the expression abundance of circCOL1A1 and miR-149-5p in the pull-down fractions was evaluated by the RT-q-PCR assay.

Wang J., Wu X., Sun X., Zhang L., Wang Q., Qu J., Wang Y, & Li Y. (2022). The Circular RNA CircCOL1A1 Functions as a miR-149-5p Sponge to Regulate the Formation of Superior-Quality Brush Hair via the CMTM3/AR Axis. Frontiers in Cell and Developmental Biology, 10, 760466.

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