The 1H-NMR spectra were recorded on a Bruker Avance III-700 spectrometer in CDCl3. Chemical shifts were referenced to the corresponding residual solvent signal (δH 7.26/δC 77.20 for CDCl3). ESI mass spectra (including HRESIMS) were obtained on a Bruker maXis Impact II LC-MS spectrometer by direct infusion in MeOH. MALDI-TOF mass spectra were obtained on a Bruker Ultraflex III TOF/TOF laser desorption spectrometer coupled with delayed extraction using a Smartbeam MALDI 200 laser with α-cyano-hydroxy cinnamic acid as the matrix. HPLC was performed on a Shimadzu instrument with a RID-10A refractive index detector using a YMC-ODS-A (250 × 10 mm) column.
Rid 10a
Manufactured by YMC
Sourced in Japan
The RID-10A is a laboratory instrument designed for the precise measurement and analysis of various samples. It features advanced sensor technology and digital processing capabilities to provide accurate and reliable results. The core function of the RID-10A is to perform quantitative analyses of samples, without interpretation or extrapolation of its intended use.
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2 protocols using rid 10a
1
NMR, Mass Spectrometry, and HPLC Analysis
2
High-Performance Liquid Chromatography for Sugars
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Glucose, fructose and sucrose were determined by a high-performance liquid chromatograph Shimadzu LC20 Prominence (Japan). The parameters of analysis of the chromatography system were set as follows: detector: refractive index RID-10A, column YMC NH 2 , 4.6 mm x 250.0 mm, 5 µm (YMC CO., LTD, Japan); temperature 35 ºC; isocratic regime; mobile phase acetonitrile (HPLC grade, Sigma-Aldrich, USA) and deionized water (Crystal E HPLC System, Adrona, Latvia) (80:20); flow rate 1.0 mL min -1 , volume of injected sample 10 µL; total time of the analysis up to 15 min. Data for analysis was processed using Shimadzu LabSolution software (LCSolution version 1.21 SP1).
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