The primary culture of human epidermal keratinocytes was kindly supported by J-TEC (Gamagori, Japan). Keratinocytes were isolated from 3 patients with each of the 3 types of SNPs in AKR1C3; rs12529 GG and rs12387 AA, rs12529 GC and rs12387 AG, and rs12529 CC and rs12387 GG. Skin tissue obtained from the patients was isolated as individual cells and proliferated according to a protocol using 3T3 cells as feeders36 (link). The completed cell lines were incubated in HuMedia-KG2 medium supplemented with 0.1% insulin, 0.1% human epidermal growth factor, 0.1% hydrocortisone, 0.4% bovine pituitary extract, and 0.1% gentamicin/amphotericin B (Kurabo, Osaka, Japan).
Gentamicin amphotericin b
Manufactured by Kurabo
Sourced in Japan
Gentamicin/amphotericin B is a laboratory product that contains the antibiotics gentamicin and amphotericin B. It is used in cell culture applications to prevent bacterial and fungal contamination.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using gentamicin amphotericin b
1
Keratinocyte Culture with AKR1C3 SNPs
2
Culturing Polarized MDCK Cell Monolayers
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Madin-Darby canine kidney cells (NBL-2) derived from dog kidney epithelial cells were obtained from the Health Research Resources Bank (Osaka, Japan) and maintained in MEM supplemented with 10% fetal calf serum and gentamicin/amphotericin B (Kurabo, Osaka, Japan) at 37°C in humidified 5% CO 2 . To pre-pare polarized MDCK cell monolayers, 0.4×10 5 trypsinized cells were seeded onto BD Falcon cell culture inserts containing 0.33 cm 2 filter membranes with 3.0 μm pores in a 24-well configuration (BD, Franklin lakes, NJ, USA). The cells were incubated until they reached confluency. Cell monolayers were used for experiments after 4 days of culture.
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