Acetylcholine perchlorate

Cover glass (no.1) for CAR measurements was purchased from VWR. The cover glass was coated with 1.5 nm Cr followed by 43 nm gold using an e-beam evaporator for SPR measurements. Dextran-coated SPR sensor chips were purchased from Biosensing Instrument Inc. (3-glycidyloxypropyl)trimethoxylsilane, N-hydroxysulfosuccinimide sodium salt (NHS), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), O-(2-Carboxyethyl)-O′-(2-mercaptoethyl)heptaethylene glycol (SH-PEG8-COOH), BSA, carbonic anhydrase lysozyme II from bovine erythrocytes (CAII), furosemide, sulpiride, methylsulfonamide, and acetylcholine perchlorate were purchased from Sigma-Aldrich. Mouse anti-BSA was purchased from MyBioSource and diluted with PBS 335 times to reach a final concentration of 20 nM. MicroRNA-21 (5′-UAGCUUAUCAGACUGAUGUUGA-3′) and biotinylated cDNA with A5 spacer (5′ biotin-AAAAATCAACATCAGTCTGATAAGCTA-3′) were purchased from Integrated DNA Technologies. Streptavidin, methyl-PEG₄-thiol (MT(PEG)4), and WGA with Alexa Fluor 488 tag were purchased from Thermo Fisher Scientific. PBS was purchased from Corning. Deionized water with a resistivity of 18.2 MΩ·cm was used in all experiments.