N nbd dppe

Manufactured by Avanti Polar Lipids

N-NBD-DPPE is a fluorescent lipid probe used in lipid research and membrane studies. It consists of a 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) molecule with a 7-nitrobenz-2-oxa-1,3-diazol-4-yl (NBD) fluorescent group attached. This probe is commonly used to investigate membrane composition, dynamics, and interactions.

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Lab products found in correlation

1 palmitoyl 2 oleoyl sn glycero 3 phospho 1 rac glycerol, by Avanti Polar Lipids (2 mentions) N rh dppe, by Avanti Polar Lipids (2 mentions) Dna primers, by Integrated DNA Technologies (1 mentions) Isopropyl β d thiogalactopyranoside iptg, by GoldBio (1 mentions) Cobalt affinity resin, by Thermo Fisher Scientific (1 mentions) N decyl β d maltopyranoside, by Anatrace (1 mentions) Isopropyl β d thiogalactopyranoside, by GoldBio (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Dipalmitoylphosphatidylethanolamine, by Avanti Polar Lipids (1 mentions)

2 protocols using n nbd dppe

Purification and Reconstitution of Membrane Proteins

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Some purchased materials were: DNA primers – Integrated DNA Technologies, Coralville, IA; Escherichia coli BL21(DE3) strain – Novagen, Gibbstown, NJ; Luria-Bertani (LB) medium – Dot Scientific, Burton, MI; isopropyl β-D-thiogalactopyranoside (IPTG) – Goldbio, St. Louis, MO; Cobalt affinity resin – Thermo Scientific, Waltham, MA; n-decyl-β-D-maltopyranoside (DM) – Anatrace, Maumee, OH; 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (sodium salt) (POPG), 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), N-(7-nitro-2,1,3-benzoxadiazol-4-yl) (ammonium salt) dipalmitoylphosphatidylethanolamine (N-NBD-DPPE), N-(lissaminerhodamine B sulfonyl) (ammonium salt) dipalmitoylphosphatidylethanolamine(N-Rh-DPPE) – Avanti Polar Lipids, Alabaster, AL. Most other materials were purchased from Sigma-Aldrich, St. Louis, MO.

Ranaweera A., Ratnayake P.U, & Weliky D.P. (2018). Stabilities of the Soluble Ectodomain and Fusion Peptide Hairpins of the Influenza Virus Hemagglutinin Subunit II Protein Are Positively-Correlated with Membrane Fusion. Biochemistry, 57(37), 5480-5493.

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Purification and Characterization of Membrane Proteins

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Commercial sources included: DNA, GenScript (Piscataway, NJ); Escherichia coli (E. coli) Novagen (Gibbstown, NJ); Luria-Bertani (LB) medium, Dot Scientific (Burton, MI); isopropyl β-D-thiogalactopyranoside (IPTG), Goldbio (St. Louis, MO); and Co²⁺ affinity resin, Thermo Scientific (Waltham, MA). Avanti Polar Lipids (Alabaster, AL) was the source for lipids that included 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (sodium salt) (POPG), N-(7-nitro-2,1,3-benzoxadiazol-4-yl) (ammonium salt) Dipalmitoylphosphatidylethanolamine} (N-NBD-DPPE) and N-(lissamine Rhodamine B sulfonyl) (ammonium salt) Dipalmitoylphosphatidylethanolamine} (N-Rh-DPPE). Other materials were typically purchased from Sigma-Aldrich (St. Louis, MO).

Rokonujjaman M., Sahyouni A., Wolfe R., Jia L., Ghosh U, & Weliky D.P. (2022). A large HIV gp41 construct with trimer-of-hairpins structure exhibits V2E mutation-dominant attenuation of vesicle fusion and helicity very similar to V2E attenuation of HIV fusion and infection and supports: (1) hairpin stabilization of membrane apposition with larger distance for V2E; and (2) V2E dominance by an antiparallel β sheet with interleaved fusion peptide strands from two gp41 trimers. Biophysical chemistry, 293, 106933.

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