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Vhx 1000 system

Manufactured by Keyence

The VHX-1000 system is a digital microscope that combines high-resolution imaging and advanced measurement capabilities. It features a variety of objective lenses, automated focus control, and integrated software for image capture and analysis.

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31 protocols using vhx 1000 system

1

Morphological Characterization of Leafhoppers

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The morphological terminology and measurements follow Bourgoin (1987) , Bourgoin (1993) and Bourgoin et al. (2015) (link) respectively for male genitalia, female genitalia and wing venation. Body length was measured from apex of vertex to tip of forewing; vertex length was measured in the median length of vertex (from apical transverse carina to tip of basal emargination). Fuchsin staining was used to highlight female genitalia structures studied. External morphology and drawings were done with the aid of a Leica MZ 12.5 stereomicroscope. Photographs were taken with KEYENCE VHX-1000 system. Illustrations were scanned with CanoScan LiDE 200 and imported into Adobe Photoshop 7.0 for labeling and plate composition. The distribution map was generated with ARCGIS 10.5. The dissected male and female genitalia are preserved in glycerin in small plastic tubes pinned together with the specimens.
The type specimens examined are deposited in the Institute of Entomology, Guizhou University, Guiyang, Guizhou Province, China (GUGC).
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2

Morphological Analysis of Insect Specimens

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The morphological terminology used in this study mainly follows Chan & Yang (1994) and Gnezdilov (2013b) . The standard terminology for hind and forewing venation follows Bourgoin et al. (2015) (link). Dry specimens were used for the descriptions and illustrations. External morphology was observed under a stereo microscope and characters were measured with an ocular micrometer. Measurements are given in millimeters; body length was measured from the apex of the head to the apex of the forewing in repose. The genital segments of the examined specimens were macerated in 10% NaOH, washed in water, and transferred to glycerin. Illustrations of the specimens were made with a Leica MZ 12.5 stereo microscope. Photographs were taken with a KEYENCE VHX-1000 system. Illustrations were scanned with CanoScan LiDE 200 and imported into Adobe Photoshop CS7 for labeling and plate composition.
The type specimens and material examined are deposited in the Institute of Entomology, Guizhou University, Guiyang, China (IEGU).
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3

Morphological Terminology for Insect Genitalia

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The morphological terminology follows Bourgoin (1987) and Bourgoin et al. (2015) (link). The morphological terminology of female genitalia follows Bourgoin (1993) . Dry specimens were used for the descriptions and illustrations. Body length was measured from the apex of the vertex to the tip of the forewing; vertex length was measured at the median length of the vertex (from the apical transverse carina to the tip of the basal emargination). Observations and drawings of external morphology were made with the aid of a Leica MZ 12.5 stereomicroscope. Photographs of the types were taken with the Keyence VHX-1000 system. Illustrations were scanned with a CanoScan LiDE 200 scanner and imported into Adobe Photoshop CS7 for labelling and plate composition. The dissected male genitalia are preserved in glycerine in small plastic tubes pinned together with the specimens.
The type specimens are deposited in the Institute of Entomology, Guizhou University, Guiyang, Guizhou Province, China (IEGU).
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4

Morphological Examination of Insect Specimens

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The morphological terminology and measurements follow Yang and Yeh (1994) and Song et al. (2014) . Specimens are deposited in the Institute of Entomology, Guizhou University, Guiyang, China (IEGU). Dry specimens were used for the observation, description, and illustration. Genital segments of the examined specimens were macerated in boiling solution of 10% potassium and drawn from preparations in glycerine jelly under a Leica MZ12.5 stereomicroscope. Color pictures for adult habitus were obtained by a KEYENCE VHX-1000 system. Illustrations were scanned with Canon Cano Scan LiDE 200 and imported into Adobe Photoshop CS6 for labelling and plate composition. The type specimens are deposited in the Institute of Entomology, Guizhou University, China (IEGU).
The following abbreviations are used in the text:
BL body length (from apex of cephalic process to tip of fore wings);
HL head length (from apex of cephalic process to base of eyes);
HW head width (including eyes);
FWL forewing length.
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5

Morphological Analysis of Insect Genitalia

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The morphological terminology adopted herein follows Nielson (1982) . Photos of external morphology were obtained by Keyence VHX-1000 system. Illustrations of male genitalia were drawn using an Olympus CX41 stereomicroscope, then enhanced by Adobe Illustrator CS6. All pictures were labelled and figs composition in Adobe Photoshop CS5. The type specimens are deposited in the Institute of Entomology, Guizhou University, Guiyang, China (GUGC).
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6

Detailed Specimen Examination Protocol

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Terminology follows Chan and Yang (1994) and Chen and Zhang (2011) (link). Dry specimens were used for the descriptions and illustrations. External morphology was observed under a stereoscopic microscope and characters were measured with an ocular micrometer. Measurements were given in millimeters; body length was measured from the apex of the head to tip of the abdomen in repose. The genital segments of the examined specimens were macerated in 10% NaOH, washed in water, and transferred to glycerin. Illustrations of the specimens were made with a Leica MZ 12.5 stereomicroscope. Photographs were taken with KEYENCE VHX-1000 system. Illustrations were scanned with CanoScan LiDE 200 and imported into Adobe Photoshop CS7 for labelling and plate composition.
The type specimens and material examined are deposited in the Institute of Entomology, Guizhou University, Guiyang, China (IEGU).
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7

Morphological and Genital Characters of Insects

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Terminology used for morphological and genital characters follow Li et al. (2011) and Zahniser and Dietrich (2013) . Leg chaetotaxy follows Dietrich (2005) . All specimens were collected by sweep net, dry male specimens were used for the description and illustration. External morphology was observed under a stereoscopic microscope and characters were measured with an ocular micrometer. Measurements are given in millimeters; body length is measured from the apex of the head to the apex of the forewing in repose. Habitus photographs were taken using a KEYENCE VHX-1000 system. The genital segments of the specimens examined were macerated in 10% NaOH and drawn from preparations in glycerin jelly using a Leica MZ 12.5 stereomicroscope. The photographs and the illustrations were scanned with Canon CanoScan LiDE 100 and imported into Adobe Photoshop CS5 for plate composition and labeling.
The type specimens examined are deposited in the Institute of Entomology, Guizhou University, Guiyang, Guizhou Province, China (IEGU) and the Natural History Museum, UK (NHMUK).
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8

Morphological Characterization of Insect Specimens

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The morphological terminology and measurements follow Yang and Yang (1986) and the morphological terminology of female genitalia follows Bourgoin (1993) . Body length was measured from apex of vertex to tip of tegmina. Dry male specimens were used for the description and illustrations. External morphology was observed under a stereoscopic microscope and characters were measured with an ocular micrometer. Color pictures for adult habitus were obtained by KEYENCE VHX-1000 system. Images of adult habitus were obtained by Canon Digital Camera EOS 5Ds. The genital segments of the examined specimens were macerated in 10% KOH and drawn from preparations in glycerin jelly using a Leica MZ 12.5 stereomicroscope. Illustrations were scanned with Canon CanoScan LiDE 200 and imported into Adobe Photoshop 6.0 for labeling and plate composition.
Specimens examined are deposited in the Institute of Entomology, Guizhou University, Guiyang, China (IEGU).
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9

Detailed Morphological Description of New Species

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The morphological terminology and measurements follow Yang and Yang (1986) . Body length was measured from apex of vertex to tip of tegmina. Dry male specimens were used for the description and illustration. External morphology was observed under a stereoscopic microscope and characters were measured with an ocular micrometer. Color pictures for adult habitus were obtained by the KEYENCE VHX-1000 system. The genital segments of the examined specimens were macerated in 10% KOH and drawn from preparations in glycerin jelly using a Leica MZ 12.5 stereomicroscope. Illustrations were scanned with a Canon CanoScan LiDE 200 and imported into Adobe Photoshop 6.0 for labeling and plate composition.
The type specimens of the new species are deposited in the Institute of Entomology, Guizhou University, Guiyang, China (IEGU).
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10

Morphological Description of Hemipteran Insects

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Terminology used for the external morphology and the male genitalia mainly follows the classifications proposed by Fennah (1987) and Chan and Yang (1994) . The standard terminology for hind and forewing venation adheres to the principle outlined by Bourgoin et al. (2015a) (link). The methodology for describing the description of the female genitalia mainly follows Bourgoin’s (1993) (link) approach. Dry specimens were used for the descriptions and illustrations. External morphology was observed under a stereoscopic microscope and characters were measured with an ocular micrometer. All dimensions were expressed in millimeters (mm); the body length was measured from the apex of the head to the apex of the forewing in repose. The genital segments of the examined specimens were subjected to maceration in a 10% NaOH and subsequently observed in glycerin jelly for illustration using a Leica MZ 12.5 stereomicroscope. Photographs were captured with a KEYENCE VHX-1000 system. Illustrations were scanned with CanoScan LiDE 200 and imported into Adobe Photoshop CS6 for labeling and plate composition. The dissected male genitalia were preserved in glycerine and then stored in small plastic tubes, which were pinned together with the specimens.
The type specimens and materials examined were deposited in the
Institute of Entomology, Guizhou University, Guiyang, China (IEGU).
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