Hth83

Human thyroid cancer epithelium cell lines (BCPAP, FTC133, 8505C, TPC-1, and HTH-83) and a normal human thyroid epithelium cell line (TEC) were commercially purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). Cells were cultured in RPMI-1640 medium (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific, Inc., Waltham, MA, USA) at 37°C in a humidified atmosphere with 5% CO₂.

Human thyroid follicular epithelial cell line Nthy-ori 3-1 and human thyroid cancer cell lines (TPC-1, HTH83, 8505C, and SW1736) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). All cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium containing 10% heat-inactivated fetal bovine serum (FBS; HyClone, Logan, UT, USA), 100 U/mL penicillin (Sigma-Aldrich, St Louis, MO, USA), and 100 μg/mL streptomycin (Sigma-Aldrich) at 37°C in a humidified incubator with 5% CO₂.
miR-141 mimic (miR-141), mimic negative control (miR-NC), miR-141 inhibitor (anti-miR-141), inhibitor scrambled control (anti-miR-NC), siRNA specifically targeting XIST (si-XIST), and scrambled negative control siRNA (si-NC) were chemically synthesized by GenePharma Co, Ltd (Shanghai, China). TPC-1 cells in logarithmic phase were transfected with these molecular products using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. Further experiments were performed at 24 h posttransfection.

Paired PTC and normal adjacent tissue samples were obtained from 47 patients who underwent surgical resection at The Third People’s Hospital of Linyi. None of these patients received any preoperative therapy, including radioiodine therapy, or chemotherapy. Following collection, all tissues were rapidly frozen in liquid nitrogen and then stored at −80°C for later use.
In total, three human PTC cell lines (TPC-1, BCPAP, and HTH83) and a normal human thyroid cell line (HT-ori3) were bought from the American Type Culture Collection (Manassas, VA, USA). All cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% heat-inactivated fetal bovine serum (FBS; Hyclone, Logan, UT, USA) and 1% antibiotic/antimycotic solution (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany). All cell lines were maintained at 37°C in a humidified incubator supplied with 5% CO₂.