Polyethylene glycol (PEG; MW: 5000 Da) linkers mPEG5k-NHS and Chelex 100 resin (50–100 mesh) were purchased from Sigma-Aldrich (St. Louis, MO). Water and all buffers were of Millipore grade and pretreated with Chelex 100 resin to ensure that the aqueous solutions were free of heavy metal. 64Cu was produced by a GE PETtrace cyclotron using the 64Ni(p,n)64Cu reaction at the institutional Cyclotron Radiochemistry Facility at The University of Texas MD Anderson Cancer Center (Houston, TX). 1,4,7-Triazacyclononane-1,4,7-triacetic acid (NOTA) was purchased from Macrocyclics, Inc. (Dallas, TX). PD SpinTrap G-25 and PD-10 desalting columns were purchased from GE Healthcare (Piscataway, NJ). Complete mouse serum was purchased from Jackson Immuno Research Laboratories (West Grove, PA). Exosome-depleted fetal bovine serum (FBS) and other chemicals and buffers were obtained from Thermo Fisher Scientific (Waltham, MA).
1 4 7 triazacyclononane 1 4 7 triacetic acid nota
Manufactured by Macrocyclics
1,4,7-Triazacyclononane-1,4,7-triacetic acid (NOTA) is a macrocyclic chelating agent. It forms stable complexes with a variety of metal ions, including radioisotopes. NOTA can be used as a component in the preparation of labeled compounds for various applications.
Automatically generated - may contain errors
Lab products found in correlation
Exosome depleted fetal bovine serum, by Thermo Fisher Scientific (1 mentions)
Complete mouse serum, by Jackson ImmunoResearch (1 mentions)
Pd 10 desalting column, by GE Healthcare (1 mentions)
Chelex 100 resin, by Merck Group (1 mentions)
Pd spintrap g 25, by GE Healthcare (1 mentions)
Pettrace cyclotron, by GE Healthcare (1 mentions)
Spintrap, by Cytiva (1 mentions)
N cyclohexyl 3 aminopropanesulfonic acid caps, by Dojindo Laboratories (1 mentions)
Proline, by Fujifilm (1 mentions)
Tyrosine, by Fujifilm (1 mentions)
Histidine, by Fujifilm (1 mentions)
Phenylalanine, by Fujifilm (1 mentions)
Tryptophan, by Fujifilm (1 mentions)
2 protocols using 1 4 7 triazacyclononane 1 4 7 triacetic acid nota
1
Radiolabeling of Antibodies with 64Cu
2
Lanthanide-Based Affinity Ligand Synthesis
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All reagents employed were of analytical grade. Neu5Ac and disialic acid (diNeu5Ac) were purchased from Nagara Science (Gifu, Japan). The affinity ligands, S-2-(4-aminobenzyl)-1,4,7triazacyclononane-1,4,7-triacetic acid (ABNOTA) and 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA) were purchased from Macrocyclics (Dallas, TX). Chloride salts of lanthanides (lanthanum, europium, terbium, dysprosium, erbium, ytterbium and lutetium) were obtained from Sigma-Aldrich (St. Louis, MO). The pH buffer reagent, N-cyclohexyl-3-aminopropanesulfonic acid (CAPS), was employed (Dojindo, Kumamoto, Japan). Dimethyl sulfoxide (DMSO), glycine, proline, phenylalanine, tryptophan, histidine and tyrosine were purchased from Wako Pure Chemical Industry (Osaka, Japan). D-fructose, D-glucose and D-ribose were purchased from Kanto Kagaku (Tokyo, Japan). All stock solutions were prepared by dissolving appropriate amounts of the solid reagents into ultrapure water (>18.2 MΩ•cm), produced by Direct-Q 3UV (Nihon Millipore, Tokyo, Japan).
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