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Small rodent ventilator

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The Small Rodent Ventilator is a compact and versatile device designed for the controlled ventilation of small laboratory animals, such as rodents. The core function of this equipment is to regulate the respiratory parameters, including tidal volume and breathing rate, to support the animal's physiological needs during various research procedures.

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Lab products found in correlation

Sodium pentobarbital, by Akorn (1 mentions) Sp8 upright microscope, by Leica (1 mentions)

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Rodent ventilator (52 citations)

7 protocols using small rodent ventilator

1

Transverse Aortic Banding Protocol

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Mice were subjected to pressure overload by TAB surgery as described.[24 (link)] Briefly, mice were anesthetized with i.p. ketamine/xylazine (100 mg/kg/5 mg/kg), intubated with a 20-gauge tube and ventilated with a small rodent ventilator (Harvard Apparatus). A thoracotomy was created between the second and third intercostal space and aortic arch visualized. Aortic constriction was performed by tying a 7–0 nylon suture ligature against a 27-gauge needle to yield a narrowing 0.4 mm in diameter, with a reproducible TAB of 65–70%. In sham mice, aortic arch was visualized but not banded. The chest wall was then closed and the pneumothorax evacuated. Enzastaurin was administrated daily (i.p, 30 mg/kg/day) after TAB surgery.
Guo A., Chen R., Wang Y., Huang C.K., Chen B., Kutschke W., Hong J, & Song L.S. (2018). Transient activation of PKC results in long-lasting detrimental effects on systolic [Ca2+]i in cardiomyocytes by altering actin cytoskeletal dynamics and T-tubule integrity. Journal of molecular and cellular cardiology, 115, 104-114.
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2

Transverse Aortic Constriction Mouse Model

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Mice were anesthetized with ketamine/xylazine (100 mg/kg/5 mg/kg) via intraperitoneal injection, intubated with a 20-gauge poly-ethylene tube and ventilated with a small rodent ventilator (Harvard Apparatus, Holliston, MA). An opening was created between the second and third intercostal space where the aortic arch could be visualized. Transverse aortic constriction was performed by tightening a ligature with 7–0 PROLENE suture against a 27-gauge needle. The sham group was subjected to the same procedure but not banded. The skin was closed using 6–0 ETHILON nylon sutures. Mice were then removed from the ventilator, extubated and anesthesia stopped. Following recovery, mice were administered buprenorphine (1 mg/kg subcutaneous injection) twice a day for 48 hours.
Zhang Y., Taufalele P.V., Cochran J.D., Frayne I.R., Marx J.M., Soto J., Rauckhorst A.J., Tayyari F., Pewa A.D., Gray L.R., Teesch L.M., Puchalska P., Funari T.R., McGlauflin R., Zimmerman K., Kutschke W.J., Cassier T., Hitchcock S., Lin K., Kato K.M., Stueve J.L., Haff L., Weiss R.M., Cox J.E., Rutter J., Taylor E.B., Crawford P.A., Lewandowski E.D., Des Rosiers C, & Abel E.D. (2020). Mitochondrial Pyruvate Carriers are Required for Myocardial Stress Adaptation. Nature metabolism, 2(11), 1248-1264.
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3

Transverse Aortic Constriction Mouse Model

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Mice were anesthetized with ketamine/xylazine (100 mg/kg/5 mg/kg) via intraperitoneal injection, intubated with a 20-gauge poly-ethylene tube and ventilated with a small rodent ventilator (Harvard Apparatus, Holliston, MA). An opening was created between the second and third intercostal space where the aortic arch could be visualized. Transverse aortic constriction was performed by tightening a ligature with 7–0 PROLENE suture against a 27-gauge needle. The sham group was subjected to the same procedure but not banded. The skin was closed using 6–0 ETHILON nylon sutures. Mice were then removed from the ventilator, extubated and anesthesia stopped. Following recovery, mice were administered buprenorphine (1 mg/kg subcutaneous injection) twice a day for 48 hours.
Zhang Y., Taufalele P.V., Cochran J.D., Frayne I.R., Marx J.M., Soto J., Rauckhorst A.J., Tayyari F., Pewa A.D., Gray L.R., Teesch L.M., Puchalska P., Funari T.R., McGlauflin R., Zimmerman K., Kutschke W.J., Cassier T., Hitchcock S., Lin K., Kato K.M., Stueve J.L., Haff L., Weiss R.M., Cox J.E., Rutter J., Taylor E.B., Crawford P.A., Lewandowski E.D., Des Rosiers C, & Abel E.D. (2020). Mitochondrial Pyruvate Carriers are Required for Myocardial Stress Adaptation. Nature metabolism, 2(11), 1248-1264.
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4

Myocardial Infarction Induction in Mice

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Male EP3 TG mice or WT controls at 10–12 weeks of age were anesthetized with sodium pentobarbital (50 mg x kg−1) (Akorn, Inc., Lake Forest, IL. USA), intubated, and ventilated with a small rodent ventilator (Harvard Apparatus, Holliston, MA, USA) at a rate of 90 cycles min−1 with a tidal volume of 0.5 ml and a positive end-expiratory pressure of 2 cmH2O. A left side thoracotomy was performed, and the pericardium was incised. Myocardial infarction was then induced through permanent ligation of the left anterior descending coronary artery using 8–0 silk suture proximal to its bifurcation from the main stem. Sham surgery was performed as described above except that the suture was not tied around the artery. The chest incision was subsequently closed with a 4–0 silk suture. Mice recovered in a temperature-controlled environment. After surgery, mice were given a dose of buprenorphine (0.05 mg x kg−1 s.c.) every 12 h for 2 days. All studies involving the use of animals were approved by the animal care and use committee (IACUC) at Henry Ford Hospital and Wayne State University, in accordance with federal guidelines.
Maxwell D.L., Bryson T.D., Taube D., Xu J., Peterson E, & Harding P. (2022). Deleterious Effects of Cardiomyocyte-Specific Prostaglandin E2 EP3 Receptor Overexpression on Cardiac Function After Myocardial Infarction. Life sciences, 313, 121277.
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5

In Vivo Imaging of Mouse Lung Microvasculature

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Mice were anesthetized [xylazine hydrochloride (10 mg/kg) and ketamine hydrochloride (200 mg/kg)], and the body temperature was maintained at 37°C using a heating pad (CU-201, Live Cell Instrument). The right jugular vein was cannulated to administer fluorescent dyes and additional anesthetic. The trachea of the mouse was exposed, and a small catheter was threaded into the trachea. The catheter was then attached to a small rodent ventilator (Harvard Apparatus). The mouse was placed on its right lateral decubitus position. A small surgical incision was made, and the intercostal muscles between ribs 4 and 5 were gently teased apart forming a ~1.5-cm opening. Intercostal lung window was then carefully placed between ribs 4 and 5. The lung was stabilized with a suction of ~20 mmHg. Images were acquired with a Leica Sp8 upright microscope equipped with a resonance scanner.
Thanabalasuriar A., Chiang A.J., Morehouse C., Camara M., Hawkins S., Keller A.E., Koksal A.C., Caceres C.S., Berlin A.A., Holoweckyj N., Takahashi V.N., Cheng L., de los Reyes M., Pelletier M., Patera A.C., Sellman B., Hess S., Marelli M., Boo C.C., Cohen T.S, & DiGiandomenico A. (2021). PD-L1+ neutrophils contribute to injury-induced infection susceptibility. Science Advances, 7(10), eabd9436.
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6

Transverse Aortic Constriction Mouse Model

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The pressure overload phenotype in mice was induced by TAC, as described.76 Briefly, mice were anesthetised with inhalational isoflurane (2%–3%), intubated orally with a 20‐gauge tube, and ventilated with a small rodent ventilator (Harvard Apparatus, Holliston, MA). Respiratory rate and body temperature were monitored continuously during the procedure. A thoracotomy was created between the second and third intercostal space, and the aortic arch was visualised. Aortic constriction was performed by tying a 7‐0 Prolene suture ligature against a 27‐gauge needle, yielding a 0.4‐mm diameter when the needle was removed. In sham mice, the aortic arch was visualised but not constricted.
Gaddam R.R., Kim Y., Jacobs J.S., Yoon J., Li Q., Cai A., Shankaiahgari H., London B., Irani K, & Vikram A. (2022). The microRNA‐204‐5p inhibits APJ signalling and confers resistance to cardiac hypertrophy and dysfunction. Clinical and Translational Medicine, 12(1), e693.
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7

Transverse Aortic Banding in Mice

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Male and female mice aged 10–12 wks were subjected to cardiac pressure overload by transverse aortic banding (TAB) as described [40 (link)]. Briefly, mice were anesthetized with ketamine/xylazine (40/5 mg/kg, respectively) by intraperitoneal injection. The mice were intubated with a 16 gauge tube, and ventilated with a small rodent ventilator (Harvard Apparatus, Holliston, MA). A thoracotomy was created between the second and third intercostal space, and the aortic arch visualized. TAB was performed by placing a suture around the aorta and the shank of an 18-gauge needle. The needle was then removed. In sham operated animals, the aortic arch was visualized but not banded. The chest wall was closed, and the pneumothorax evacuated.
Gao Z., Sierra A., Zhu Z., Koganti S.R., Subbotina E., Maheshwari A., Anderson M.E., Zingman L.V, & Hodgson-Zingman D.M. (2016). Loss of ATP-Sensitive Potassium Channel Surface Expression in Heart Failure Underlies Dysregulation of Action Potential Duration and Myocardial Vulnerability to Injury. PLoS ONE, 11(3), e0151337.
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