Human pancreatic cells were dissociated from surgical pancreatic tissues using a Tumor Dissociation Kit (human) and gentleMACS Dissociator (Miltenyi Biotec, Bergisch-Gladbach, Germany) according to the manufacturer's instructions. Immediately after dissociation, the cells were analyzed by flow cytometry. In addition, we analyzed the following nine pancreatic cancer cell lines: BxPC3, CFPac-1, SW1990, AsPC1, and Capan-2 (American Type Culture Collection, Manassas, Va); Panc-1 (RIKEN, Tsukuba, Japan); MiaPaCa2, SUIT-2, and KP-2 (Health Science Research Resources Bank, Osaka, Japan). We also analyzed two normal pancreatic duct epithelial cell lines: a human primary normal pancreatic epithelial cell line, CS-PE (DS Pharma Biomedical Co., Osaka, Japan), and an immortalized pancreatic ductal epithelial cell line, HPDE6-E6E7 clone 6 (a gift from Dr. Ming-Sound Tsao, University of Toronto, Toronto, Canada). In addition, human pancreatic stellate cells (PSCs) were isolated from fresh pancreatic specimens using the outgrowth method [14] (link). The metastatic SUIT-2 cell line was previously established in our laboratory [15] (link). The same method was used to establish the metastatic Panc-1 cell line. Cells were maintained as described previously [16] (link).
Miapaca2
Manufactured by Health Science Research Resources Bank
Sourced in Japan
MiaPaCa2 is a cell line derived from a human pancreatic carcinoma. It is a commonly used in vitro model for studying pancreatic cancer biology and evaluating potential therapeutic interventions.
Automatically generated - may contain errors
Lab products found in correlation
Suit 2, by Health Science Research Resources Bank (2 mentions)
Aspc 1, by American Type Culture Collection (2 mentions)
Sw1990, by American Type Culture Collection (2 mentions)
Capan 2, by American Type Culture Collection (2 mentions)
Gentlemacs dissociator, by Miltenyi Biotec (1 mentions)
Bxpc 3, by American Type Culture Collection (1 mentions)
Cfpac 1, by American Type Culture Collection (1 mentions)
Tumor dissociation kit, by Miltenyi Biotec (1 mentions)
Capan 1, by American Type Culture Collection (1 mentions)
Hs766t, by American Type Culture Collection (1 mentions)
2 protocols using miapaca2
1
Isolation and Characterization of Pancreatic Cell Types
2
Isolation and Characterization of Human Pancreatic Stellate Cells
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Human PSCs were isolated from fresh pancreatic surgical specimens using the outgrowth method described by Bachem et al.27 , 28 The PSC cell type was confirmed by morphology (stellate‐like or spindle‐shaped cells) and by immunofluorescence staining for α‐smooth muscle actin (α‐SMA) and vimentin.28 , 29 , 30 The use of pancreatic cancer surgical specimens was approved by the Ethics Committee of Kyushu University (Fukuoka, Japan) and was carried out according to the Ethical Guidelines for Human Genome/Gene Research enacted by the Japanese Government and the Helsinki Declaration. We used 10 human PCC cell lines in this study: PANC‐1 (Institute of Physical and Chemical Research, Saitama, Japan), KP‐2 and KP‐3 (Japan Health Sciences Foundation, Tokyo, Japan), SUIT‐2 and MIA PaCa‐2 (Japan Health Science Research Resources Bank, Osaka, Japan), and AsPC‐1, CAPAN‐1, CAPAN‐2, Hs766T, and SW1990 (ATCC, Manassas, VA, USA), as well as normal human pancreatic epithelial cells (CS‐PE; Cell Systems, Applied Cell Biology Research Institute, Kirkland, WA, USA). The HPDE cell line was kindly provided by Dr. Ming‐Sound Tsao (University of Toronto, Toronto, ON, Canada). All of the cells were maintained as previously described.31
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