The HCT116 CRC cell line is purchased from the American Type Culture Collection (Manassas,VA). HCT116 cells were cultured in RPMI 1640 medium (Thermo Fisher Scientific). The medium was supplemented with 10% fetal bovine serum (FBS) (Gibco, Gaithersburg, MD) and 1% penicillin − streptomycin (Hyclone, Logan, UT), and the cell lines were maintained at 37 °C in a humidified atmosphere consisting of 5% CO2 and 95% air.
Hct116 crc cell line
Manufactured by American Type Culture Collection
The HCT116 CRC cell line is a commonly used colon cancer cell line derived from a human colorectal carcinoma. It is a robust, well-characterized model for studying various aspects of colorectal cancer biology.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Hct116, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Nacalai Tesque (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Biowest (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Ccd 18co normal colon fibroblasts, by American Type Culture Collection (1 mentions)
3 protocols using hct116 crc cell line
1
HCT116 CRC Cell Culture Protocol
2
Culturing Endothelial and Colorectal Cancer Cells
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The Ea. hy926 endothelial cells (EC) and the HCT-116 CRC cell line were purchased from American Type Culture Collection (ATCC®). The cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) and Roswell Park Memorial Institute (RPMI), respectively (Thermo Scientific, United States), supplemented with 5% fetal bovine serum (Biowest, United States), 1% penicillin-streptomycin (Nacalai Tesque, United States). Cells were kept at 37oC in a humidified incubator with 5% CO2 atmosphere.
3
Cell Culture of Colorectal Cancer Lines
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HCT116 CRC cell line and CCD-18Co normal colon fibroblasts were purchased from the American Type Culture Collection. The LS174T CRC cell line was kindly provided by Dr. Ragnhild A. Lothe (Oslo University Hospital, Oslo, Norway).
HCT116 was cultured in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA); LS174T and CCD-18Co were cultured in DMEM medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). For all cell lines, the respective medium was supplemented with 10% fetal bovine serum (HyClone, Logan, UT, USA) and 1% penicillin–streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). The cells were maintained at 37 °C in a humidified atmosphere with 5% CO2.
HCT116 was cultured in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA); LS174T and CCD-18Co were cultured in DMEM medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). For all cell lines, the respective medium was supplemented with 10% fetal bovine serum (HyClone, Logan, UT, USA) and 1% penicillin–streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). The cells were maintained at 37 °C in a humidified atmosphere with 5% CO2.
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