An ovarian stimulation model was used to obtain ovaries with multiple ovulatory follicles (17 (link)). Beginning within 3 days of initiation of menstruation, monkeys received 90 IU of recombinant human follicle stimulating hormone (FSH; Merck and Co., Inc., Kenilworth, NJ) for 6–8 days, followed by 2–3 days of 90 IU of FSH plus 60 IU of recombinant human LH (Serono Reproductive Biology Institute, Rockland, MA) to stimulate the growth of multiple follicles. Animals also received a GnRH antagonist [30 μg/kg Ganirelix (Merck)] daily to prevent an endogenous ovulatory LH surge. Follicular development was monitored by ultrasonography and rising serum estradiol. During aseptic surgery, aspiration of follicles <4 mm was performed before (0), 12, 24, or 36 h after administration of 1000 IU of recombinant human chorionic gonadotropin (hCG; Serono). To inhibit follicular prostaglandin production during the periovulatory interval, some animals were treated as described above; these animals also received the PTGS2 inhibitor celecoxib (32 mg orally every 12 h; Pfizer, New York, NY) beginning with hCG administration and continuing until surgery (16 (link)). This treatment has been previously shown to significantly reduce follicular PGE2 levels (16 (link)).
Recombinant human follicle stimulating hormone
Manufactured by Merck Group
Sourced in Germany
Recombinant human follicle stimulating hormone is a laboratory product used for research purposes. It is a glycoprotein hormone that plays a role in the regulation of the reproductive system.
Automatically generated - may contain errors
2 protocols using recombinant human follicle stimulating hormone
1
Ovarian Stimulation Protocol for Multiple Follicle Growth
2
In Vitro Porcine Oocyte Production
Check if the same lab product or an alternative is used in the 5 most similar protocols
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In vitro production was conducted as described by Mito et al. [23 (link)] with slight modifications. Pig ovaries were
collected from 5–6-month-old gilts (Landrace × Large White × Duroc) bred for meat production at a slaughterhouse at our institute. Cumulus oocyte complexes (COCs) were extracted from ovaries
using a scalpel to cut the antral follicles in porcine oocyte/embryo collection medium (Research Institute for the Functional Peptides Co., Ltd.: RIFP, Yamagata, Japan) and washing multiple
times in the medium. COCs with 2–3 layers of cumulus cells were cultured in HP-POM (RIFP) with 1 mM dibutyryl cyclic AMP (dbcAMP, RIFP) and 0.5 U/ml recombinant human follicle-stimulating
hormone (Merck, Darmstadt, Germany). After 22 h of culture, COCs were transferred to HP-POM without dbcAMP and recombinant human follicle-stimulating hormone and further cultured for 24 h.
Landrace semen was purchased from Zen-Noh Livestock Co., Ltd. (Tokyo, Japan). Highly active spermatozoa were separated using a 50%/80% Percoll (GE Healthcare, Chicago, IL, USA) gradient
solution centrifuged at 700 g for 20 min, and washed twice with pig fertilization medium (RIFP) by centrifugation at 500 g for 5 min. Spermatozoa (1 ×
106/ml) were co-cultured with COCs in pig fertilization medium for 15 h. All incubations were performed at 39°C in an atmosphere of 5% CO2, 5% O2, and 90%
N2.
collected from 5–6-month-old gilts (Landrace × Large White × Duroc) bred for meat production at a slaughterhouse at our institute. Cumulus oocyte complexes (COCs) were extracted from ovaries
using a scalpel to cut the antral follicles in porcine oocyte/embryo collection medium (Research Institute for the Functional Peptides Co., Ltd.: RIFP, Yamagata, Japan) and washing multiple
times in the medium. COCs with 2–3 layers of cumulus cells were cultured in HP-POM (RIFP) with 1 mM dibutyryl cyclic AMP (dbcAMP, RIFP) and 0.5 U/ml recombinant human follicle-stimulating
hormone (Merck, Darmstadt, Germany). After 22 h of culture, COCs were transferred to HP-POM without dbcAMP and recombinant human follicle-stimulating hormone and further cultured for 24 h.
Landrace semen was purchased from Zen-Noh Livestock Co., Ltd. (Tokyo, Japan). Highly active spermatozoa were separated using a 50%/80% Percoll (GE Healthcare, Chicago, IL, USA) gradient
solution centrifuged at 700 g for 20 min, and washed twice with pig fertilization medium (RIFP) by centrifugation at 500 g for 5 min. Spermatozoa (1 ×
106/ml) were co-cultured with COCs in pig fertilization medium for 15 h. All incubations were performed at 39°C in an atmosphere of 5% CO2, 5% O2, and 90%
N2.
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