Vimentin vim
Vimentin (VIM;) is a type III intermediate filament (IF) protein that is involved in maintaining the structural integrity of cells. It plays a crucial role in the organization and dynamics of the cytoskeleton.
Lab products found in correlation
8 protocols using vimentin vim
Immunofluorescence Analysis of CAFs
Protein Extraction and Western Blot Analysis from Diverse Biological Samples
Genetically Engineered Mouse Models for Breast Cancer Research
BDH2 Overexpression in Cell Lines
BDH2 plasmid was purchased from Origene (Rockville, MD, USA); the open-reading frame of BDH2 was subcloned into the pCMV6-Entry vector. The protocol of transfection has been described.20 (link)
Molecular Techniques for Hepatocyte Analysis
Western Blot Analysis of Epithelial-Mesenchymal Transition
Molecular Techniques for Hepatocyte Analysis
kit was purchased from Qiagen (Germantown, MD). High Capacity cDNA RT kit were
purchased from ThermoFisher Scientific (Waltham, MA). RT2 SYBR Green
ROX qPCR Mastermix and primers for Gapdh were purchased from Qiagen (Valencia,
CA). Primary antibodies against Alb, hypoxia inducing factor 1 alpha
(Hif-1α), and Vimentin (Vim) were purchased from Cell signaling (Danvers,
MA). Primary antibodies against nuclear factor-like 2 (Nrf2) and 3-Oxoacid CoA
Transferase 1 (Oxctl) were purchased from Abeam (Cambridge, MA). Primary
antibody against Fas was purchased from SantaCruz Biotechnology (SantaCruz, CA).
Primary antibody against NeuN was purchased from Millipore (Billerica, MA).
U-PLEX combo kit against TNF-α was purchased from Meso Scale Diagnostics
(Rockville, MD).
Immunofluorescence Staining of Tumor Cells
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