NR4A1 antibody was purchased from Abcam (Cambridge, MA), and TXNDC5, PGK1 and ATP5A1 antibodies were purchased from GeneTex (Irvine, CA). XBP-1s (spliced XBP-1) and phosphor-PERK (Ser713) antibodies were purchased from BioLengend (San Diego, CA, USA). ATF4, CHOP, GRP78, β-actin, and IDH1 antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA), and ATF6 andibody was purchased from Abgent (Atlanta, GA, USA). All remaining antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). DIM-C-pPhOH was synthesized and purified in this laboratory as previously described (19 (link)). Reporter lysis buffer, luciferase reagent, and β-galactosidase (β-gal) reagent were supplied by Promega (Madison, WI, USA). Quantitative real-time PCR and western blot analysis were undertaken as previously described (20 (link)). The real-time PCR primers for NR4A1 were purchased from Qiagen (Valencia, CA, USA), and all other sequences of the primers used for real-time PCR were shown in Supplemental Table 1 .
Nr4a1 antibody
Manufactured by Abcam
The NR4A1 antibody is a tool used in research applications to detect and study the NR4A1 protein, which is a transcription factor that plays a role in various cellular processes. The antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to identify and quantify the NR4A1 protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Luciferase reagent, by Promega (1 mentions)
Reporter lysis buffer, by Promega (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Atp5a1, by GeneTex (1 mentions)
β galactosidase β gal reagent, by Promega (1 mentions)
Enhanced chemiluminescence detection kit, by Merck Group (1 mentions)
Polyvinylidene fluoride membrane, by Bio-Rad (1 mentions)
β actin, by Proteintech (1 mentions)
Fbw7 antibody, by Fortis Life Sciences (1 mentions)
Scd1 antibody, by Cell Signaling Technology (1 mentions)
2 protocols using nr4a1 antibody
1
Protein Expression Analysis in Cells
2
Comprehensive Western Blot Analysis
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Western blotting was carried out as previously described [5 (link)]. Briefly, whole-cell protein lysates were extracted and then were separated by SDS-PAGE and blotted onto polyvinylidene fluoride membranes (Bio-Rad). After blocking, the membranes were incubated with corresponding antibodies: FBW7 antibody (Bethyl), SCD1 antibody (Cell Signaling Technology), NR4A1 antibody (Abcam), NR4A2 antibody (Abcam), β-actin (Proteintech). Next, the membranes were probed with secondary antibodies conjugated to HRP (Proteintech). Finally, immunoblots were incubated with an enhanced chemiluminescence detection kit (Millipore) and visualized by imaging systems (CLiNX).
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