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Human il 6 and il 8 elisa kits

Manufactured by R&D Systems
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The Human IL-6 and IL-8 ELISA kits are laboratory tools used for the quantitative measurement of human interleukin-6 (IL-6) and interleukin-8 (IL-8) levels in biological samples such as cell culture supernatants, serum, and plasma. These kits utilize the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify the target analytes.

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Lab products found in correlation

Protein lysis buffer, by Thermo Fisher Scientific (2 mentions) Non targeting control sirna, by Horizon Discovery (1 mentions) Rabbit monoclonal anti phospho erk1 2, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

IL-6 and IL-8 ELISA kits Human IL-8 ELISA kit Human IL-6 ELISA kit IL-8 ELISA kit IL-6 and IL-8 IL-6 kits Human IL-8 Human IL-6 Human kits ELISAs

6 protocols using human il 6 and il 8 elisa kits

1

Quantification of Human IL-6 and IL-8

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Human IL-6 and IL-8 ELISA kits (R&D systems, Minneapolis, USA) were used, according to the instructions of manufacturer. Briefly, cancer cells were treated with CMs for 4 days, then cells were washed twice with PBSx1 and incubated in DMEM serum-free media for 24 h. Complete supernatant was collected, (centrifuged at 120× g for 5 min at RT) and processed for ELISA.
Odeh A., Eddini H., Shawasha L., Chaban A., Avivi A., Shams I, & Manov I. (2023). Senescent Secretome of Blind Mole Rat Spalax Inhibits Malignant Behavior of Human Breast Cancer Cells Triggering Bystander Senescence and Targeting Inflammatory Response. International Journal of Molecular Sciences, 24(6), 5132.
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2

MUC1 Immunodetection Assay Protocol

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All reagents were purchased from Sigma (St. Louis, MO) unless noted otherwise. Anti-MUC1 mouse monoclonal antibody (GP1.4, Cat. # MA5–13168), Alexa Fluor 488-conjugated rabbit anti-mouse or goat anti-rabbit IgG, and Alexa Fluor 594-conjugated rabbit anti-mouse or goat anti-rabbit IgG were from Thermo Fisher Scientific (Waltham, MA). Polyclonal rabbit anti-mouse MUC1 antibody was from Abcam (Cat. # ab15481, Cambridge, MA). Horseradish peroxidase (HRP)-conjugated goat anti-mouse and goat anti-rabbit IgG antibodies were from Jackson ImmunoResearch Laboratories, Inc (West Grove, PA). The pCI-MUC1 expression plasmid and pCI-neo empty vector were a generous gift from Dr. Sandra J. Gendler (Mayo Clinic, Scottsdale, AZ). MUC1 smart pool siRNA and non-targeting control siRNA were purchased from Dharmacon (Lafayette, CO). Rabbit monoclonal anti-total ERK1/2, rabbit monoclonal anti-phospho-ERK1/2, and HRP-conjugated mouse monoclonal anti-beta actin antibodies were purchased from Cell Signaling (Danvers, MA). Polyclonal mouse anti-major surface glycoprotein (Msg) of Pneumocystis murina (P. murina) was generated as previously described (Bishop, Helman, & Kovacs, 2012 (link)). Human IL-6 and IL-8 ELISA kits were from R&D (Minneapolis, MN).
Liu Y., Davis A.S., Ma L., Bishop L., Cissé O.H., Kutty G, & Kovacs J.A. (2020). MUC1 Mediates Pneumocystis murina Binding to Airway Epithelial Cells. Cellular microbiology, 22(6), e13182.
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3

Quantifying Cytokine Responses to UVA in HSFs

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HSFs were grown in 24-well plates, pretreated with CeO2 NP for 24 h, and exposed to UVA radiation. Then, culture supernatants were collected and examined using human IL-6 and IL-8 ELISA kits (R&D Systems, Minneapolis, MN, USA) according to manufacturer instructions. Concentrations of IL-6 and IL-8 were obtained using standard curves.
Li Y., Hou X., Yang C., Pang Y., Li X., Jiang G, & Liu Y. (2019). Photoprotection of Cerium Oxide Nanoparticles against UVA radiation-induced Senescence of Human Skin Fibroblasts due to their Antioxidant Properties. Scientific Reports, 9, 2595.
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4

Assessing Cytokine Secretion in A549 Cells

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A549 cells were transfected with MUC1 siRNA or control siRNA for 48 hours, followed by serum starvation for 24 hours, after which the cells were incubated with or without Pneumocystis for 16 hours, in serum-free medium. Cell culture supernatants were then harvested and stored at −70°C. IL-6 and IL-8 levels in supernatants were determined using human IL-6 and IL-8 ELISA kits (R&D) per the manufacturer’s instructions; the detection sensitivity is 0.7 pg/ml and 7.5 pg/ml respectively. The experiment was performed in triplicate.
Liu Y., Davis A.S., Ma L., Bishop L., Cissé O.H., Kutty G, & Kovacs J.A. (2020). MUC1 Mediates Pneumocystis murina Binding to Airway Epithelial Cells. Cellular microbiology, 22(6), e13182.
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5

Inflammatory Cytokine Secretion in IECs

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IEC in culture were stimulated with 1ng/mL of IL-1β after 48 hrs of fatty acid supplementation. Six hrs after IL-1β exposure, cell free supernatant (CFS) from IEC in culture were collected, gently vortexed and frozen immediately in aliquots at −80° C for IL-8 and IL-6 analysis. IL-8 and IL-6 concentration in CFS was determined by ELISA using human IL-8 and IL-6 Elisa kits (R&D Systems, Minneapolis, MN). Protein content of IEC in culture were determined by lysing cells in monolayer with protein lysis buffer (Thermo Scientific, Rockford, IL) and measuring protein content of cell lysate by nanometer. IL-8 and IL-6 concentration in CFS was expressed per mg protein in cell lysates (ng/mg protein).
Wijendran V., Brenna J., Wang D.H., Zhu W., Meng D., Ganguli K., Kothapalli K.S., Requena P., Innis S, & Walker W. (2015). Long chain poly-unsaturated fatty acids attenuate the IL-1β-induced pro-inflammatory response in human fetal intestinal epithelial cells. Pediatric research, 78(6), 626-633.
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6

Inflammatory Cytokine Secretion in IECs

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IEC in culture were stimulated with 1ng/mL of IL-1β after 48 hrs of fatty acid supplementation. Six hrs after IL-1β exposure, cell free supernatant (CFS) from IEC in culture were collected, gently vortexed and frozen immediately in aliquots at −80° C for IL-8 and IL-6 analysis. IL-8 and IL-6 concentration in CFS was determined by ELISA using human IL-8 and IL-6 Elisa kits (R&D Systems, Minneapolis, MN). Protein content of IEC in culture were determined by lysing cells in monolayer with protein lysis buffer (Thermo Scientific, Rockford, IL) and measuring protein content of cell lysate by nanometer. IL-8 and IL-6 concentration in CFS was expressed per mg protein in cell lysates (ng/mg protein).
Wijendran V., Brenna J., Wang D.H., Zhu W., Meng D., Ganguli K., Kothapalli K.S., Requena P., Innis S, & Walker W. (2015). Long chain poly-unsaturated fatty acids attenuate the IL-1β-induced pro-inflammatory response in human fetal intestinal epithelial cells. Pediatric research, 78(6), 626-633.
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