Methyl imidazole (Sigma Aldrich, 99%), 1-bromo butane (C4H9Br), (Sigma Aldrich 99%), toluene (C6H5CH3) pub chem, titanium isopropoxide (Ti[OCH(CH3)2]4) Sigma Aldrich, ammonium heptamolybdate ((NH4)2MoO4) 99.98% trace metals basis, nitric acid (60–79%), Thomos baker. Dolo (paracetamol) 650 mg. Acetic Acid (99%) and sodium acetate (99%) (Thomos baker). All additional chemicals were of analytical quality, and double-distilled water was used to make all solutions.
Methylimidazole
Manufactured by Merck Group
Sourced in India
Methylimidazole is a chemical compound that is used as a laboratory reagent. It has the molecular formula C₄H₆N₂. Methylimidazole is a colorless to pale yellow liquid with a characteristic odor. It is soluble in water and organic solvents.
Automatically generated - may contain errors
Lab products found in correlation
1 4 butane sultone, by Merck Group (3 mentions)
Hexane, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Titanium isopropoxide, by Merck Group (1 mentions)
Toluene, by Merck Group (1 mentions)
1 bromobutane, by Merck Group (1 mentions)
Triethylamine, by Merck Group (1 mentions)
Sylgard 184, by Dow (1 mentions)
Zinc nitrate hexahydrate, by Merck Group (1 mentions)
Cetrimonium bromide ctab, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
2 propanol, by Merck Group (1 mentions)
4 vinylbenzyl chloride, by Merck Group (1 mentions)
Castor oil, by SD Fine Chemicals (1 mentions)
Sodium hydroxide (naoh), by SD Fine Chemicals (1 mentions)
H2so4, by SD Fine Chemicals (1 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride, by Merck Group (1 mentions)
Perfecthyb plus hybridization buffer liquid, by Merck Group (1 mentions)
Hybond, by Cytiva (1 mentions)
Agcf3so3, by Merck Group (1 mentions)
10 protocols using methylimidazole
1
Synthesis of Imidazolium-based Materials
2
Synthesis of ZIF-L Membrane
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Polyethersulfone Ultrason E6020P (PES) and two components Dow Corning Sylgard 184 (PDMS) were used to fabricate the membranes in this work. n-pentane, n-heptane and 1-methyl-2-pyrrolidone which are the solvents used to prepare the membrane were supplied by Merck. Meanwhile, methylimidazole (Sigma Aldrich), zinc nitrate hexahydrate (Sigma Aldrich), triethylamine (Merck) and deionized water were used to synthesize ZIF-L, according to the procedure by Khan et al. [9 (link)].
3
Fabrication of Polymer Films and Membranes
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Poly(ethylene terephthalate) dense films (Mylar®) were purchased from Chemplex Industries, Inc., and 4-inch diameter silicon wafers and glass wafers were bought from University Wafer; cetrimonium bromide (CTAB), zinc acetate dihydrate (Zn(CH3COO)2·2H2O, >99%) and methylimidazole were supplied by Sigma-Aldrich. All soft stamp materials were provided by EVG: working stamp material UV/AF1 (fluoropolymer), photoinitiator, anti-sticking (methyl fluorobutyl ether) and adhesion agents (isopropyl alcohol). Track-etched membranes were purchased from STERLITECH Corporation, with pore sizes of 400 nm and 200 nm.
4
Jatropha Seed Extraction Protocol
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Local dried Jatropha seeds were collected from the Kaliabor Nursery, Kaliabor, Assam, India. 1-Methylimidazole (99%), 1,4-butane sultone (≥99%), and 2-propanol (anhydrous, 99.5%) were procured from Sigma Aldrich, India. Methanol (≥99%, GC grade), hexane (fraction from petroleum), HCl (35% for analysis), and KOH (analytical grade) were purchased from Merck India Limited. All the chemicals were used as received without any further purification.
5
Synthesis of Imidazolium Cationic Surfactants
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MA used in the reaction
was of HPLC grade. Azobisisobutyronitrile (AIBN) (98%), which was
recrystallized using MA, was purchased from Avra Synthesis. Castor
oil, NaOH (60% suspension in paraffin oil), 98% concentrated H2SO4, and benzimidazole were obtained from SD Fine
Chemicals. 1,4-Butane sultone, methyl imidazole, and 4-vinylbenzyl
chloride (90%) were purchased from Sigma-Aldrich, India.
was of HPLC grade. Azobisisobutyronitrile (AIBN) (98%), which was
recrystallized using MA, was purchased from Avra Synthesis. Castor
oil, NaOH (60% suspension in paraffin oil), 98% concentrated H2SO4, and benzimidazole were obtained from SD Fine
Chemicals. 1,4-Butane sultone, methyl imidazole, and 4-vinylbenzyl
chloride (90%) were purchased from Sigma-Aldrich, India.
6
Northern Blot Analysis of RNA Samples
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Northern blot was performed as described [35 , 84 ]. Total RNA was separated on 14% denaturing urea-polyacrylamine gels and run with 0.5 × TBE at 150 V. The gel was transferred to Hybond membrane NX (GE healthcare, RPN303T) at 14 V overnight. Chemical crosslink buffer was prepared as follows: 0.373 g N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (Sigma-Aldrich, E7550), 3 drops of 1 M HCl, 121 µl Methylimidazole (Sigma-Aldrich, M50834), and 12 mL RNase-free H2O. After chemical crosslink at 60 °C for 2 h and UV crosslink at 85 °C for 2 h, the membrane was pre-incubated with PerfectHyb™ Plus Hybridization Buffer liquid (Sigma-Aldrich, H7033) for 30 min, then hybridized overnight at 37 °C with γ- 32P ATP (China isotope & radiation corporation) labelled DNA probes by T4-polynucleotide kinase (NEB, M0201S) for 4 h. After that, the membrane was washed with buffer contains 2 × SSC and 0.025% SDS. Auto-radiography of the membrane was performed using a Typhoon Scanner. Sequences of probes are listed in Supplementary Table S4 .
7
Organometallic Protein Interaction Assay
8
Synthesis of 1-Butylimidazole from Imidazole
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Imidazole (Him), methyl Imidazole (mim), butyl bromide, 1,4butanesultone, sodium ethoxide, p-nitrophenyl acetate, sulfuric acid (97-98%), and sodium bisulfate used as precursors or reactants were from Sigma-Aldrich. Methanol, sulfuric ether, ethyl acetate, hexane, acetone, butyl alcohol, and dichloromethane used as solvents or eluents were from Sigma-Aldrich. 1-ButylImidazole (bim) was synthesized by reaction of Imidazole with butyl bromide through an aliphatic nucleophilic substitution, following a previously reported method that employs sodium ethoxide for deprotonating the Imidazole, 27, 28 as it described in the ESI. †
9
Synthesis and Characterization of Metal-Organic Catalysts
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Zinc nitrate hexahydrate (98%, Zn(NO3)2·6H2O) and ferric nitrate nonahydrate (98%, Fe(NO3)3·9H2O) were purchased from Alfa Aesar. Methanol and ethanol were purchased from Sinopharm. 2-Methylimidazole (99%), superoxide dismutase (≥2500 units/mg protein), and xanthine oxidase (from milk, ≥0.4 units/mg protein) were obtained from Sigma-Aldrich. PBS was obtained from YoBiBio. Catalase (from bovine liver, 20000 units/mg) was obtained from Adamas. Catalase Assay Kit and Total superoxide dismutase Kit were purchased from Shanghai Beyotime Biotechnology Co., Ltd. Ascorbic acid, xanthine (98%), hydrogen peroxide (H2O2, 30 wt%), and sulfuric acid (98%) were purchased from Aladdin Co. Ltd. 5-tert-butoxycarbonyl 5-methyl-1-pyrroline N-oxide (BMPO) were purchased from Shanghai Dojindo Co. Ltd.
10
Synthesis and Characterization of Coumarin Derivatives
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4-Hydroxy coumarin,
substituted aromatic aldehydes, 2-amino pyridine, methylimidazole,
2-chloro acetic acid, and sodium tetrafluoroborate were obtained from
Sigma-Aldrich, and organic solvents were procured from commercial
suppliers and used without any further purification. Analytical thin-layer
chromatography (TLC) was carried out using 0.25 mm silica gel-coated
Kieselgel 60 F254 plates. 1H NMR (400 MHz), 13C NMR (100 MHz), 19F NMR (100 MHz), and 10B
NMR (128 MHz) spectra were measured on a Bruker DRX400 spectrometer.
Chemical shifts and coupling constants are specified in parts per
million (ppm) and Hertz (Hz), respectively, using tetra-methyl silane
as an internal standard and the solvent resonance at (DMSO-d6: 1H NMR 400 MHz and 13C NMR 100 MHz): δ
2.49 and 39.7 ppm. The peak multiplicities are allocated as s (singlet),
d (doublet), dd (doublet of doublet), dt (doublet of triplet), and
m (multiplet). FT-IR spectra were recorded using a IRAffinity (SHIMADZU)
and Nicolet iS50 (Thermo Scientific) spectrometer on a KBr disc. UV–Vis
absorption spectra were recorded on a JASCO V-670 spectrophotometer
at room temperature in acetonitrile. A Hitachi F-7000 FL spectrophotometer
was utilized to record emission fluorescence spectra by excitation
at their respective absorption maxima.
substituted aromatic aldehydes, 2-amino pyridine, methylimidazole,
2-chloro acetic acid, and sodium tetrafluoroborate were obtained from
Sigma-Aldrich, and organic solvents were procured from commercial
suppliers and used without any further purification. Analytical thin-layer
chromatography (TLC) was carried out using 0.25 mm silica gel-coated
Kieselgel 60 F254 plates. 1H NMR (400 MHz), 13C NMR (100 MHz), 19F NMR (100 MHz), and 10B
NMR (128 MHz) spectra were measured on a Bruker DRX400 spectrometer.
Chemical shifts and coupling constants are specified in parts per
million (ppm) and Hertz (Hz), respectively, using tetra-methyl silane
as an internal standard and the solvent resonance at (DMSO-d6: 1H NMR 400 MHz and 13C NMR 100 MHz): δ
2.49 and 39.7 ppm. The peak multiplicities are allocated as s (singlet),
d (doublet), dd (doublet of doublet), dt (doublet of triplet), and
m (multiplet). FT-IR spectra were recorded using a IRAffinity (SHIMADZU)
and Nicolet iS50 (Thermo Scientific) spectrometer on a KBr disc. UV–Vis
absorption spectra were recorded on a JASCO V-670 spectrophotometer
at room temperature in acetonitrile. A Hitachi F-7000 FL spectrophotometer
was utilized to record emission fluorescence spectra by excitation
at their respective absorption maxima.
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