Quanta 3d feg dual beam esem

For morphological analysis of the cell attached scaffolds, the samples were washed with PBS after removing the medium and fixed with 0.6 ml of 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer solution for 30 min at 4 °C. After thorough washing twice with PBS, scaffolds were dehydrated through a series of graded ethanol concentrations (30%, 50%, 70%, 90% and 100%) and finally dried in HMDS. After proper drying, scaffolds were coated with gold and palladium using a sputter coater (Cressington 108 Auto) and were then observed using an SEM (FEI Quanta 3D FEG dual beam ESEM, USA) operated at an accelerating voltage of 5 kV.

The surface morphology of both porous and non-porous scaffolds was examined using the secondary electron detector of scanning electron microscopy (SEM) (FEI Quanta 3D FEG dual beam ESEM, USA) followed by copper sputter-coating at accelerating voltage of 5 keV. EDX analysis was done for elemental mapping of scaffolds using backscattered electron detector of SEM followed by gold-palladium sputter-coating at accelerating voltage of 20 keV.

For morphological analysis of the cell attached scaffolds, the samples were washed with PBS after removing the medium and fixed with 0.6 ml of 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer solution for 30 min at 4 °C. After thorough washing twice with PBS, scaffolds were dehydrated through a series of graded ethanol concentrations (30%, 50%, 70%, 90% and 100%) and finally dried in HMDS. After proper drying, scaffolds were coated with gold and palladium using a sputter coater (Cressington 108 Auto) and were then observed using an SEM (FEI Quanta 3D FEG dual beam ESEM, USA) operated at an accelerating voltage of 5 kV.