Human il8 elisa max deluxe set

Manufactured by BioLegend

Sourced in United States

The Human IL8 ELISA MAX™ Deluxe Set is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human interleukin-8 (IL-8) in cell culture supernatants, serum, and plasma.

Automatically generated - may contain errors

Lab products found in correlation

Elisa max deluxe set human ccl2, by BioLegend (2 mentions) Elisa max deluxe set human il 6, by BioLegend (2 mentions) Human cxcl10 elisa max deluxe set, by BioLegend (2 mentions) Human cxcl1 duo set kit, by R&D Systems (2 mentions) Ultra low attachment plate, by Corning (1 mentions) Elisa max deluxe set human il 1β, by BioLegend (1 mentions) Human il 6 elisa max deluxe, by BioLegend (1 mentions) Human tnf α elisa max deluxe, by BioLegend (1 mentions) Elisa max deluxe set human il 1α, by BioLegend (1 mentions) Golgistop, by BD (1 mentions) Radioimmunoprecipitation assay buffer, by Beyotime (1 mentions) Elisa max standard set human il 6, by BioLegend (1 mentions) Human myeloperoxidase duoset elisa, by R&D Systems (1 mentions) Polyinosinic polycytidylic acid, by Merck Group (1 mentions) Human ccl20, by R&D Systems (1 mentions) Cxcl1, by R&D Systems (1 mentions) Bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Human il 29 il 28b, by R&D Systems (1 mentions) Mouse cxcl1 kc duoset elisa kit, by R&D Systems (1 mentions) Human cxcl1 gro alpha duoset elisa, by R&D Systems (1 mentions)

12 protocols using human il8 elisa max deluxe set

Cytokine Profiling of Stressed Human Islets

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pancreatic islets were obtained from human organ donor pancreata. Human islets were isolated from organ donors. Islets were only studied if they could not be used for clinical purposes and if research consent had been obtained. According to the national law ethics approval is not required for research on donor tissues that cannot be used for clinical transplantation. The isolations were performed in the GMP-facility of LUMC. For experimental use, human islets were maintained in ultra-low attachment plates (Corning, NY 14831) in low glucose DMEM supplemented with 10% FBS, 100 units/ml Penicillin and 100 μg/ml streptomycin and exposed to 1µM thapsigargin for 5h. After treatment, culture medium was replaced and analyzed for cytokine profile 2 days after using Luminex 9-plex kit (BioRad) according to the manufacturer’s protocol. Islet cells were pelleted and RNA isolated to correct Luminex values. Human IL8 secretion from EndoC-βH1 cells exposed to TG was determined by ELISA MAX™ Deluxe Set Human IL-8 (Biolegend) according to the protocol from the manufacturer.

Vig S., Lambooij J.M., Dekkers M.C., Otto F., Carlotti F., Guigas B, & Zaldumbide A. (2022). ER stress promotes mitochondrial DNA mediated type-1 interferon response in beta-cells and interleukin-8 driven neutrophil chemotaxis. Frontiers in Endocrinology, 13, 991632.

+ Open protocol

+ Expand

Quantifying Cytokine Levels in PBMC

Check if the same lab product or an alternative is used in the 5 most similar protocols

The amount of cytokines (CCL2, CXCL8, and Il6) in treated PBMC supernatants were quantified by ELISA using the ELISA MAX Deluxe Set Human CCL2 (Biolegend), ELISA MAX Deluxe Set Human IL8 (Biolegend), and ELISA MAX Deluxe Set Human IL6 (Biolegend) kits according to the manufacturer’s protocol.

Santoso C.S., Li Z., Rottenberg J.T., Liu X., Shen V.X, & Fuxman Bass J.I. (2021). Therapeutic Targeting of Transcription Factors to Control the Cytokine Release Syndrome in COVID-19. Frontiers in Pharmacology, 12, 673485.

+ Open protocol

+ Expand

Cytokine and Neurotransmitter Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Culture supernatants were collected after centrifugation at 5,000 rpm for 5 min and stored at −80°C. For intracellular measurements, cells were treated by Golgistop (554724; BD Biosciences) for 4 h and then lysed by radioimmunoprecipitation assay buffer (P0013B; Beyotime Biotechnology). IL-6, TNF-α, IL-1α, IL-1β, IL-8, and CXCL10 were measured by Human IL-6 ELISA MAX Deluxe (430505; BioLegend), Human TNF-α ELISA MAX Deluxe (430205; BioLegend), ELISA MAX Deluxe Set Human IL-1α (445804; BioLegend), ELISA MAX Deluxe Set Human IL-1β (437004; BioLegend), ELISA MAX Deluxe Set Human IL-8 (431504; BioLegend), and LEGEND MAX Human CXCL10 (IP-10) ELISA Kit (439907; BioLegend) according to the manufacturers’ instructions. Adrenaline and noradrenaline were measured by the human adrenaline ELISA kit (MM-0751H; MEIMIAN) and human noradrenaline ELISA kit (MM-0995H; MEIMIAN), according to the manufacturer’s instructions.
For cAMP detection, cells were lysed by cell lysis buffer provided by the cAMP Direct Immunoassay Detection Kit (Fluorometric; ab138880; Abcam), and cAMP was measured according to the manufacturer’s instructions.

Yang Y., Zhang Y., Xing X., Xu G., Lin X., Wang Y., Chen M., Wang C., Zhang B., Han W, & Hu X. (2023). IL-6 translation is a therapeutic target of human cytokine release syndrome. The Journal of Experimental Medicine, 220(11), e20230577.

+ Open protocol

+ Expand

Quantifying Cytokine Levels in PBMC

Check if the same lab product or an alternative is used in the 5 most similar protocols

Santoso C.S., Li Z., Rottenberg J.T., Liu X., Shen V.X, & Bass J.I. (2020). In vitro Targeting of Transcription Factors to Control the Cytokine Release Syndrome in COVID-19. bioRxiv.

+ Open protocol

+ Expand

Quantifying Inflammatory Markers in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

At 6 h post-gentamicin treatment, cell supernatants were harvested and IL-8 (ELISA Max Deluxe Set Human IL-8, Biolegend), IL-6 (ELISA Max Standard Set Human IL-6, Biolegend), and MPO (Human Myeloperoxidase DuoSet ELISA, R&D systems) levels quantfied following the manufacturer’s guidelines.

Vozza E.G., Daly C.M., O'Rourke S.A., Fitzgerald H.K., Dunne A, & McLoughlin R.M. (2023). Staphylococcus aureus suppresses the pentose phosphate pathway in human neutrophils via the adenosine receptor A2aR to enhance intracellular survival. mBio, 15(1), e02571-23.

+ Open protocol

+ Expand

Poly(I:C) Stimulation Induces IL-8 Secretion

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were tested after their second passage and all experiments were conducted in triplicate. Briefly, 7,200 cells were seeded per well in a 96-well plate in 50 μL of KSFM. After 24 hours, 50 μL of KSFM containing 20 μg/mL of polyinosinic:polycytidylic acid (poly(I:C); Sigma-Aldrich) was added in half of the wells to reach a final concentration of 10 μg/mL, while 50 μL of KSFM alone was added to control wells. After 20 hours, cell supernatants were collected for IL-8 measurement and total DNA was measured according to the DRAQ5^® LI-COR^® protocol (LI-COR Biosciences, Lincoln, NE, USA), to account for the number of cells present per well. IL-8 secretion was measured in the culture medium using the human IL-8 ELISA MAX™ Deluxe set (BioLegend, San Diego, CA, USA), as per the manufacturer’s instructions. With the exception of the time course experiment, IL-8 secretion was normalized to total DNA per well.

Neveu B., Moreel X., Deschênes-Rompré M.P., Bergeron A., LaRue H., Ayari C., Fradet Y, & Fradet V. (2014). IL-8 secretion in primary cultures of prostate cells is associated with prostate cancer aggressiveness. Research and Reports in Urology, 6, 27-34.

+ Open protocol

+ Expand

Quantifying Cytokine Secretion in Colonoids

Check if the same lab product or an alternative is used in the 5 most similar protocols

After 4 h stimulation of colonoids in Matrigel dome, the media was collected and centrifuged at 1000 g at 4 °C for 10 min, then the supernatant was collected and stored at − 80 °C. 100 uL of the supernatant (or dilution) was used per well in duplicate and the ELISAs were performed according to manufacturer’s instructions (human CCL20 (R&D systems), human IL-8 ELISA MAX Deluxe Set (BioLegend), murine Ccl20 and Cxcl1 (R&D systems)).

Allaire J.M., Poon A., Crowley S.M., Han X., Sharafian Z., Moore N., Stahl M., Bressler B., Lavoie P.M., Jacobson K., Li X, & Vallance B.A. (2021). Interleukin-37 regulates innate immune signaling in human and mouse colonic organoids. Scientific Reports, 11, 8206.

+ Open protocol

+ Expand

Quantifying IL8 in Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Collected culture conditioned media and cells lysates were centrifuged, supernatants were collected and total protein content was determined by BCA™ Protein Assay Kit (Thermo Scientific Pierce). The IL8 content in culture media was determined following the protocol of Human IL8 ELISA MAX Deluxe Set (BioLegend, San Diego, CA) with detection limit of 15.6 pg/ml and sensitivity of 8 pg/ml. The level of IL8 was reported as pg/mg of protein or as fold change compared to control experiments.

Chuang T.D., Rehan A, & Khorram O. (2020). Tranilast Induces MiR-200c Expression through Blockade of RelA/p65 Activity in Leiomyoma Smooth Muscle Cells. Fertility and sterility, 113(6), 1308-1318.

+ Open protocol

+ Expand

Quantifying IL-8 Secretion in HT-29 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

For analysis of IL-8 secretion, HT-29 cell monolayers were infected for 3 h before being incubated for 8-12 h in media supplemented with 50 μg/mL gentamicin with or without 20 ng/mL TNF (Calbiochem, EMD4Biosciences, USA). Following this, the HT-29 cell supernatant was collected and either used immediately or stored at -20 °C for subsequent analysis of IL-8 secretion. IL-8 secretion was measured using the Human IL-8 ELISA MAX Deluxe Set (Biolegend, CA, USA) according to the manufacturer’s instructions.

Pearson J.S., Giogha C., Mühlen S., Nachbur U., Pham C.L., Zhang Y., Hildebrand J.M., Oates C.V., Lung T.W., Ingle D., Dagley L.F., Bankovacki A., Petrie E.J., Schroeder G.N., Crepin V.F., Frankel G., Masters S.L., Vince J., Murphy J.M., Sunde M., Webb A.I., Silke J, & Hartland E.L. (2017). EspL is a bacterial cysteine protease effector that cleaves RHIM proteins to block necroptosis and inflammation. Nature microbiology, 2, 16258.

+ Open protocol

+ Expand

Quantifying Protein Levels in HBEC and BAL Fluid

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein levels in the supernatants of HBECs or BAL fluid were analyzed by ELISA. For HBEC samples, human IL‐8 ELISA MAX Deluxe Set (BioLegend, 431504), human CXCL1/GRO alpha DuoSet ELISA (R&D Systems, DY275‐05), and human IL‐29/IL‐28B (IFN‐lambda 1/3) DuoSet ELISA kit (R&D Systems, DY1598B‐05) were used according to the manufacturers’ instructions. For BAL fluids, a mouse CXCL1/KC DuoSet ELISA kit (R&D Systems, DY453‐05) was used as instructed by the manufacturer. The data were acquired with a Ledetect 96 Microplate reader (Labexim Products, Lengau, Austria). Online analysis software Myassays.com and a four‐parameter logistic regression model were used to calculate the concentrations of the proteins.

Laanesoo A., Urgard E., Periyasamy K., Laan M., Bochkov Y.A., Aab A., Magilnick N., Pooga M., Gern J.E., Johnston S.L., Coquet J.M., Boldin M.P., Wengel J., Altraja A., Bochenek G., Jakiela B, & Rebane A. (2021). Dual role of the miR‐146 family in rhinovirus‐induced airway inflammation and allergic asthma exacerbation. Clinical and Translational Medicine, 11(6), e427.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!