The cell lines used are human cancer cell lines: A2780 (ovarian carcinoma), HT29 (colon adenocarcinoma), MCF-7 (breast adenocarcinoma), A375 (melanoma), FaDu (pharynx squamous cell carcinoma) and non-malignant mouse fibroblasts NIH 3T3; all cell lines were obtained from the Department of Oncology (Martin-Luther-University Halle-Wittenberg). Cultures were maintained as monolayers in RPMI 1640 medium with l -glutamine (Capricorn Scientific GmbH, Ebsdorfergrund, Germany) supplemented with 10% heat inactivated fetal bovine serum (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) and penicillin/streptomycin (Capricorn Scientific GmbH, Ebsdorfergrund, Germany) at 37 °C in a humidified atmosphere with 5% CO2.
Rpmi 1640 medium with l glutamine
Manufactured by Capricorn
Sourced in Germany
RPMI 1640 medium with l-glutamine is a cell culture medium formulated to support the growth of a variety of mammalian cell lines. It contains essential amino acids, vitamins, and other nutrients required for cell proliferation. The inclusion of l-glutamine provides a source of energy and carbon for cells.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Penicillin streptomycin, by Capricorn (2 mentions)
Hct116, by Thermo Fisher Scientific (1 mentions)
Skov3, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Mcf 7, by Thermo Fisher Scientific (1 mentions)
Panc 1, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Capricorn (1 mentions)
Doxorubicin hcl, by Merck Group (1 mentions)
L glutamine, by Capricorn (1 mentions)
3 protocols using rpmi 1640 medium with l glutamine
1
Culturing Human Cancer Cell Lines
2
Cytotoxicity Evaluation of Cancer Cell Lines
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Following human cancer cell lines A375 (malignant melanoma), HT29 (colon adenocarcinoma), MCF-7 (breast cancer), A2780 (ovarian carcinoma), FaDu (pharynx carcinoma), and non-malignant mouse fibroblasts NIH 3T3 were used. All cell lines were obtained from the Department of Oncology (Martin-Luther-University Halle Wittenberg). Cultures were maintained as monolayers in RPMI 1640 medium with L-glutamine (Capricorn Scientific GmbH, Ebsdorfergrund, Germany) supplemented with 10% heat-inactivated fetal bovine serum (Sigma-Aldrich GmbH, Steinheim, Germany) and penicillin/streptomycin (Capricorn Scientific GmbH, Ebsdorfergrund, Germany) at 37°C in a humidified atmosphere with 5% CO2.
3
Cytotoxicity Evaluation of Cell Lines
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The following materials were used in the project and were purchased from the corresponding companies. Fetal bovine serum (HyClone, Logan, UT), HEPES buffer (HyClone), McCoy's 5a Medium Modified (HyClone), Dulbecco's modified Eagle medium (DMEM) high glucose medium with l-glutamine (Capricorn, Ebsdorfergrund, Germany), DMEM Hams F12 medium with l-glutamine (Capricorn), RPMI 1640 medium with l-glutamine (Capricorn), Matrix 96-Well Tissue Culture Treated Plates (Sigma-Aldrich, St. Louis, MO), l-glutamine (Capricorn), sterile syringe filter pore 0.22 μm (Millipore, Billerica, MA), doxorubicin HCl (Sigma-Aldrich), PBS (Capricorn), the tetrazolium dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) kit (Promega, Madison, WI), and dimethyl sulfoxide (Gibco, Waltham, MA).
The tested cell lines and their ATCC numbers (in parentheses) are as follows: HCT116 (CCL247), MCF-7 (HTB22), K562 (CCL243), A549 (CCL185), PANC1 (CRL1469), PC3 (CRL1435), and SKOV3 (HTB77).
The tested cell lines and their ATCC numbers (in parentheses) are as follows: HCT116 (CCL247), MCF-7 (HTB22), K562 (CCL243), A549 (CCL185), PANC1 (CRL1469), PC3 (CRL1435), and SKOV3 (HTB77).
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