RNA samples were lysed in TRIzol (Invitrogen) and then reverse transcribed using the TaqMan RT-qPCR kit according to manufacturer’s instructions by ARQ Genetics. TaqMan fluorescent probes for mTOR and GAPDH were used and mRNA expression was quantified using ΔΔCt comparison normalized to the GAPDH control.
Taqman rt qpcr kit
Manufactured by Thermo Fisher Scientific
The TaqMan RT-qPCR kit is a molecular biology product designed for reverse transcription and real-time quantitative PCR (RT-qPCR) analysis. It includes reagents necessary for the conversion of RNA to cDNA and the subsequent amplification and detection of target sequences.
Automatically generated - may contain errors
Lab products found in correlation
Trizol, by Thermo Fisher Scientific (1 mentions)
Steponeplus real time pcr system, by Thermo Fisher Scientific (1 mentions)
Iscript cdna synthesis kit, by Bio-Rad (1 mentions)
Peqgold trifast, by Avantor (1 mentions)
Mirvana mirna isolation kit, by Thermo Fisher Scientific (1 mentions)
Itaq sybr green supermix with rox, by Bio-Rad (1 mentions)
Taqman universal pcr master mix, by Thermo Fisher Scientific (1 mentions)
Turbo dna free kit, by Thermo Fisher Scientific (1 mentions)
2 protocols using taqman rt qpcr kit
1
Quantifying mTOR mRNA Expression
2
RNA Isolation and Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total RNA was isolated using mirVana miRNA isolation kit (AM1561, Invitrogen) or peqGOLD Trifast (30‐2010, VWR) for RNA immunoprecipitation. Genomic DNA contamination was eliminated with TURBO DNA‐free kit (AM1907, Invitrogen). Reverse transcription of RNA was carried out using iScript cDNA synthesis kit (170‐8891, Bio‐Rad) or TaqMan RT–qPCR kit (4366597, Invitrogen), according to manufacturer's protocols. Relative cDNA quantification was performed using StepOnePlus Real‐Time PCR system (Applied Biosystems) using iTaq SYBR Green Supermix with ROX (172‐5121, Bio‐Rad) for detection of precursor miRNAs and TaqMan Universal PCR Master Mix (4364341, Invitrogen) for detection of mature miRNAs.
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