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Plck plyn

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PLCK/pLYN is a laboratory equipment product. It performs core functions related to sample processing and analysis. No further details are available while maintaining an unbiased and factual approach.

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Lab products found in correlation

2 7 dichlorofluorescin diacetate dcfda, by Abcam (2 mentions) Pplcγ2, by BD (2 mentions) Pacific blue conjugated anti cd3 clone ucht1, by BD (2 mentions) Percpcy5.5 conjugated anti cd20 clone h1, by BD (2 mentions) Pe cy7 conjugated anti cd5 clone l17f12, by BD (2 mentions) Shp 1 antibody, by Santa Cruz Biotechnology (2 mentions) Matlab, by MathWorks (2 mentions) Lsrii cytometer, by BD (2 mentions)

2 protocols using plck plyn

1

Multiparametric Flow Cytometry for CLL

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies with the following specificities were purchased from BD Biosciences: pPLCγ2 (Alexa Fluor 488, pY759), pSYK (PE, pY348), pLCK/pLYN (Alexa Fluor 647, pY505). Phosphatase abundance was estimated by SHP-1 antibody (Santa Cruz Biotechnology). Detection of PBMC subsets was achieved with Pacific Blue-conjugated anti-CD3 (clone UCHT1) and PerCPCy5.5 conjugated anti-CD20 (clone H1), and CLL circulating tumor was confirmed for CD5 positivity with PE-Cy7-conjugated anti-CD5 (clone L17F12, all BD Biosciences). Intracellular reactive oxygen species was measured under resting and H2O2-stimulated conditions using 2′,7’-dichlorofluorescin diacetate (DCFDA) (Abcam). For assessment of intracellular species, cells were permeabilized following fixation with PFA with cold methanol at −20°C for 10 minutes. Intracellular staining was carried out at 4°C in PBS + 2% FBS. Cells were washed twice with PBS + 2% FBS and analyzed on an LSRII cytometer (BD Biosciences). Analysis was carried out using FlowJo software (TreeStar), Prism (Graphpad), and with custom tools written in R statistical computing language (R project) and MATLAB (Mathworks). Code is available upon request.
Ziegler C.G., Kim J., Piersanti K., Oyler-Yaniv A., Argyropoulos K.V., van den Brink M.R., Palomba M.L., Altan-Bonnet N, & Altan-Bonnet G. (2019). Constitutive Activation of the B Cell Receptor Underlies Dysfunctional Signaling in Chronic Lymphocytic Leukemia. Cell reports, 28(4), 923-937.e3.
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2

Multiparametric Flow Cytometry for CLL

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies with the following specificities were purchased from BD Biosciences: pPLCγ2 (Alexa Fluor 488, pY759), pSYK (PE, pY348), pLCK/pLYN (Alexa Fluor 647, pY505). Phosphatase abundance was estimated by SHP-1 antibody (Santa Cruz Biotechnology). Detection of PBMC subsets was achieved with Pacific Blue-conjugated anti-CD3 (clone UCHT1) and PerCPCy5.5 conjugated anti-CD20 (clone H1), and CLL circulating tumor was confirmed for CD5 positivity with PE-Cy7-conjugated anti-CD5 (clone L17F12, all BD Biosciences). Intracellular reactive oxygen species was measured under resting and H2O2-stimulated conditions using 2′,7’-dichlorofluorescin diacetate (DCFDA) (Abcam). For assessment of intracellular species, cells were permeabilized following fixation with PFA with cold methanol at −20°C for 10 minutes. Intracellular staining was carried out at 4°C in PBS + 2% FBS. Cells were washed twice with PBS + 2% FBS and analyzed on an LSRII cytometer (BD Biosciences). Analysis was carried out using FlowJo software (TreeStar), Prism (Graphpad), and with custom tools written in R statistical computing language (R project) and MATLAB (Mathworks). Code is available upon request.
Ziegler C.G., Kim J., Piersanti K., Oyler-Yaniv A., Argyropoulos K.V., van den Brink M.R., Palomba M.L., Altan-Bonnet N, & Altan-Bonnet G. (2019). Constitutive Activation of the B Cell Receptor Underlies Dysfunctional Signaling in Chronic Lymphocytic Leukemia. Cell reports, 28(4), 923-937.e3.
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