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MIFNβ is a laboratory reagent produced by Cell Signaling Technology. It functions as a cytokine that plays a role in the immune response.

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Lab products found in correlation

Nitrocellulose membrane, by Merck Group (2 mentions) Enhanced chemi luminescence (ecl), by Thermo Fisher Scientific (2 mentions) Amicon ultra 10 kda, by Merck Group (2 mentions) Ponceau s solution, by Merck Group (2 mentions) Peroxidase linked secondary antibody, by Cell Signaling Technology (2 mentions)

2 protocols using mifnβ

1

Immunoblotting of IFNβ and TRAIL

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell culture supernatant was concentrated using Amicon Ultra 10 kDa (Sigma) and these protein samples were electrophoresed on 4–20% SDS-polyacrylamide gels and transferred to nitrocellulose membrane (Merck Millipore). The membranes were incubated with primary antibodies against mIFNβ (#97450) and TRAIL (#3219) (Cell Signaling Technology), followed by peroxidase-linked secondary antibody (#7074, Cell Signaling Technology). The membrane was probed with secondary antibodies and developed with ECL (Thermo Fisher Scientific). Equal loading of the supernatant samples was confirmed with the Ponceau S solution (Sigma).
van Schaik T.A., Moreno-Lama L., Farzani T.A., Wang M., Chen K.S., Li W., Cai L., Zhang Y.S, & Shah K. (2023). Engineered cell-based therapies in ex vivo ready-made CellDex capsules have therapeutic efficacy in solid tumors. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 162, 114665.
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2

Immunoblotting of IFNβ and TRAIL

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell culture supernatant was concentrated using Amicon Ultra 10 kDa (Sigma) and these protein samples were electrophoresed on 4–20% SDS-polyacrylamide gels and transferred to nitrocellulose membrane (Merck Millipore). The membranes were incubated with primary antibodies against mIFNβ (#97450) and TRAIL (#3219) (Cell Signaling Technology), followed by peroxidase-linked secondary antibody (#7074, Cell Signaling Technology). The membrane was probed with secondary antibodies and developed with ECL (Thermo Fisher Scientific). Equal loading of the supernatant samples was confirmed with the Ponceau S solution (Sigma).
van Schaik T.A., Moreno-Lama L., Farzani T.A., Wang M., Chen K.S., Li W., Cai L., Zhang Y.S, & Shah K. (2023). Engineered cell-based therapies in ex vivo ready-made CellDex capsules have therapeutic efficacy in solid tumors. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 162, 114665.
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