Kinetic colorimetric assay kit

Manufactured by Thermo Fisher Scientific

The Kinetic colorimetric assay kit is a laboratory tool designed to measure the concentration of a specific analyte in a sample over time. It utilizes a colorimetric reaction to determine the analyte's concentration quantitatively.

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Lab products found in correlation

Mitoprofile rapid microplate assay kit, by Abcam (3 mentions) Mitochondria isolation kit, by Solarbio (2 mentions) Bca assay, by Beyotime (2 mentions) Atp assay kit, by Beyotime (1 mentions)

3 protocols using kinetic colorimetric assay kit

Mitochondrial Function Assay Protocol

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Mitochondria were isolated from VSMCs using Mitochondria Isolation Kits (Solarbio, China) according to the manufacturer’s protocol. The activities of complexes I, II, III, and IV were measured with the MitoProfile Rapid Microplate Assay Kit (Abcam) according to the manufacturer’s instructions. Complexes I, II, III, and IV were immune-captured in the wells in the microplates, and enzymatic activity was measured with a kinetic colorimetric assay kit (Thermo Fisher Scientific). The total and mitochondrial protein concentrations were determined using the BCA assay (Beyotime).

Ma D., Zheng B., Liu H.L., Zhao Y.B., Liu X., Zhang X.H., Li Q., Shi W.B., Suzuki T, & Wen J.K. (2020). Klf5 down-regulation induces vascular senescence through eIF5a depletion and mitochondrial fission. PLoS Biology, 18(8), e3000808.

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Mitochondrial Enzyme Activity Assay

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Mitochondria were isolated from gastrocnemius or C2C12 cells using Mitochondria Isolation Kits (Solarbio, China) according to the manufacturer's protocol. The activities of complexes I, II, IV and ATP synthase (V) were measured with the MitoProfile Rapid Microplate Assay Kit (Abcam) according to the manufacturer's instructions. Complexes I, II, IV, and V were immune-captured in the wells of microplates, and enzymatic activity was measured with a kinetic colorimetric assay kit (Thermo Fisher Scientific). The total and mitochondrial protein concentrations were determined using the BCA assay (Beyotime).

Yang X., Xue P., Chen H., Yuan M., Kang Y., Duscher D., Machens H.G, & Chen Z. (2020). Denervation drives skeletal muscle atrophy and induces mitochondrial dysfunction, mitophagy and apoptosis via miR-142a-5p/MFN1 axis. Theranostics, 10(3), 1415-1432.

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Measuring Mitochondrial Enzyme Activity

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ATP levels were measured using the ATP Assay Kit (Beyotime, China) according to manufacturer's protocol. The activity of complex I, II, IV and V in hippocampus homogenates (50 g of total protein) was measured with a MitoProfile Rapid Micro-plate assay kit according to the manufacturer's instructions (catalog nos. ab109721, ab109908 and ab109911, respectively, Abcam, USA). Complexes I, II, IV and V were immune-captured within the wells of the microplates and enzyme activity was measured by a kinetic colorimetric assay kit (Thermo Fisher Scientific Inc.) as described by the manufacturer. Total protein in hippocampus homogenates was estimated using a protein assay kit (DC, Bio-Rad, USA).

Liu P., Zou D., Yi L., Chen M., Gao Y., Zhou R., Zhang Q., Zhou Y., Zhu J., Chen K, & Mi M. (2015). Quercetin ameliorates hypobaric hypoxia-induced memory impairment through mitochondrial and neuron function adaptation via the PGC-1α pathway. Restorative neurology and neuroscience, 33(2).

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