8 oh dg

Manufactured by Elabscience

Sourced in United States, China

8-OH-dG is a nucleoside that is formed when the DNA base guanine is oxidized by reactive oxygen species. It is commonly used as a biomarker for oxidative stress and DNA damage.

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Lab products found in correlation

Tnf α, by Elabscience (2 mentions) Elisas, by Thermo Fisher Scientific (1 mentions) Interleukin 6 (il 6), by Elabscience (1 mentions) Caspase 3, by Elabscience (1 mentions)

6 protocols using 8 oh dg

Cytokine and DNA Damage Assays

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IL-1α and TGF-β were measured in cell-free supernatants obtained from the PBMCs culture, respectively, after 5 and 24 h of treatment, using commercially available enzyme-linked immunosorbent assay kits (ELISAs) (eBioscience, CA, United States; R&D Systems, United States).
The level of 8-OH-dG was measured following manufacturer’s instructions (Elabscience, Houston, TX United States) after 1 h of treatment.

Colarusso C., Terlizzi M., Molino A., Imitazione P., Somma P., Rega R., Saccomanno A., Aquino R.P., Pinto A, & Sorrentino R. (2019). AIM2 Inflammasome Activation Leads to IL-1α and TGF-β Release From Exacerbated Chronic Obstructive Pulmonary Disease-Derived Peripheral Blood Mononuclear Cells. Frontiers in Pharmacology, 10, 257.

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Cytokine and Oxidative Stress ELISA

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ELISA kits of IL-6, TNF-α, and 8-OHDG were purchased from Elabscience (Wuhan, China). Experiments were conducted following the ELISA kit protocol. The standard curve and sample wells were prepared separately as instructed, and the absorbance of each well was measured at 450nm. Each sample was represented in triplicates. Standard curves were plotted, and R² greater than 0.99 was considered reliable and used for calculating concentration.

Hong Y., Li J., Zhong Y., Yang S., Pei L., Huang Z., Chen X., Wu H., Zheng G., Zeng C., Wu H, & Wang T. (2023). Elabela inhibits TRAF1/NF-κB induced oxidative DNA damage to promote diabetic foot ulcer wound healing. iScience, 26(9), 107601.

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Ovarian Oxidative and Inflammatory Biomarkers

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About 0.1 g of the right ovaries of each animal were homogenized on ice in 10 ml of cold phosphate buffer solution. The resultant solutions were centrifuged at 10,000 rpm for15 mins in cold centrifuge after which the supernatant was decanted into tubes and stored at −20°C for biochemical assay later.
The ovarian concentrations of UA [26 (link)], MDA [27 (link)], and GSH [28 (link)] were assayed by colorimetric method as previously reported. The activities of XO [27 (link)], superoxide dismutase (SOD) [28 (link)], catalase [28 (link)], glutathione peroxidase (GPx) [29 (link)], glutathione-S-transferase (GST) [27 (link)], and myeloperoxidase (MPO) [29 (link)] in the ovarian tissues were assayed by colorimetry as earlier documented. Also, the ovarian levels of TNF-α (Elabscience Biotechnology Inc., U.S.A.), IL-6 (Elabscience Biotechnology Inc., U.S.A.), 8OHdG (Elabscience Biotechnology Inc., U.S.A.), and VCAM-1 (Elabscience, Wuhan, China) as well as the activity of caspase 3 (Elabscience Biotechnology Inc., U.S.A.) were determined using ELISA kit following the manufacturers’ guidelines. Ovarian DNA fragmentation index was determined by diphenylamine methods as previously reported [25 ].

Afolabi O.A., Hamed M.A., Anyogu D.C., Adeyemi D.H., Odetayo A.F, & Akhigbe R.E. (2022). Atorvastatin-mediated downregulation of VCAM-1 and XO/UA/caspase 3 signaling averts oxidative damage and apoptosis induced by ovarian ischaemia/reperfusion injury. Redox Report : Communications in Free Radical Research, 27(1), 212-220.

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COPD Cytokine and Oxidative Stress Assay

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IL-18 and IL-33 were measured in cell-free supernatants (75 × 10⁴ cells/well) using commercially available ELISA kits (eBioscience, CA, USA). No differences in cytokine release were observed according to stage of COPD patients. 8-OH-dG was measured following manufacturer’s instructions after 3 h of treatment (5 × 10⁶ cells/well) (Elabscience, USA). The release of caspase-4 was analyzed by an ELISA kit patented by ImmunePharma s.r.l. (RM2014A000080 and PCT/IB2015/051262) (Department of Pharmacy, University of Salerno, Italy). According to the patent policy, and because it is still not commercially available, it is not at the moment possible to describe the technical approach to determine the release of caspase-4 by using the ImmunePharma’s ELISA kit.

De Falco G., Colarusso C., Terlizzi M., Popolo A., Pecoraro M., Commodo M., Minutolo P., Sirignano M., D’Anna A., Aquino R.P., Pinto A., Molino A, & Sorrentino R. (2017). Chronic Obstructive Pulmonary Disease-Derived Circulating Cells Release IL-18 and IL-33 under Ultrafine Particulate Matter Exposure in a Caspase-1/8-Independent Manner. Frontiers in Immunology, 8, 1415.

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Biomarker Assessment in Biological Processes

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A total of 8 biomarkers of interest under different categories of biological process were investigated in this study, including antioxidant and oxidative damage [including superoxide dismutase (SOD) activity, malondialdehyde (MDA), and 8-hydroxy-2’-deoxyguanosine (8-OHdG)], inflammation [C-reactive protein (CRP), interleukin 6 (IL-6), and tumor necrosis factor α (TNF-α)], nutrient metabolism [vitamin D], and genomic stability [telomerase]. Commercially available metabolism assay kits that were used for the detection of SOD activity and MDA levels, as well as the ELISA kits that were used for the detection of 8-OHdG, CRP, IL-6, TNF-α, vitamin D, and telomerase, were all purchased from Elabscience Biotechnology Co., Ltd. (Wuhan, China). The metabolism assays and the ELISA tests were conducted based on the protocol stated in the user manual.

Ooi T.C., Ahmad A., Rajab N.F, & Sharif R. (2023). The Effects of 12 Weeks Colostrum Milk Supplementation on the Expression Levels of Pro-Inflammatory Mediators and Metabolic Changes among Older Adults: Findings from the Biomarkers and Untargeted Metabolomic Analysis. Nutrients, 15(14), 3184.

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Cardiac Tissue Biomarker Quantification

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Spectrophotometric methods using standard laboratory reagents were used to determine the cardiac tissue levels of MDA [20 (link)], GSH [21 (link)], and nitric oxide (NO) [22 (link)].
Standard ELISA kits were used for the in vitro quantitative determination of 8-hydroxydeoxyguanosine (8-OHdG) (Cat#E-EL-0028) Collagen type III (COL3) (Cat#E-EL-R0235) and vascular endothelial growth factor (VEGF-A) (Cat#E-EL-R 2603), and procured from Elabscience, Wuhan, China.

Mowaad N.A., Elgohary R, & ElShebiney S. (2024). Effect of Stanozolol and/or Cannabis Abuse on Hypertrophic Mechanism and Oxidative Stress of Male Albino Rat Cardiac Tissue in Relation to Exercise: A Sport Abuse Practice. Cardiovascular Toxicology, 24(6), 527-538.

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